Please use this identifier to cite or link to this item: https://doi.org/10.1038/srep12554
Title: Identification of a novel lymphoid population in the murine epidermis
Authors: Almeida, F.F
Tenno, M
Brzostek, J 
Li, J.L
Allies, G
Hoeffel, G
See, P
Ng, L.G 
Fehling, H.J
Gascoigne, N.R.J 
Taniuchi, I
Ginhoux, F 
Keywords: CD2 antigen
CD3 antigen
gamma interferon
homeodomain protein
interleukin 2
lymphocyte antigen receptor
RAG-1 protein
Thy 1 antigen
animal
C57BL mouse
cell culture
cell differentiation
coculture
cytology
epidermis
genetics
immunology
knockout mouse
metabolism
mouse
multiphoton microscopy
nude mouse
phenotype
T lymphocyte
Animals
Antigens, CD2
Antigens, CD3
Antigens, Thy-1
Cell Differentiation
Cells, Cultured
Coculture Techniques
Epidermis
Homeodomain Proteins
Interferon-gamma
Interleukin-2
Mice
Mice, Inbred C57BL
Mice, Knockout
Mice, Nude
Microscopy, Fluorescence, Multiphoton
Phenotype
Receptors, Antigen, T-Cell, gamma-delta
T-Lymphocytes
Issue Date: 2015
Publisher: Nature Publishing Group
Citation: Almeida, F.F, Tenno, M, Brzostek, J, Li, J.L, Allies, G, Hoeffel, G, See, P, Ng, L.G, Fehling, H.J, Gascoigne, N.R.J, Taniuchi, I, Ginhoux, F (2015). Identification of a novel lymphoid population in the murine epidermis. Scientific Reports 5 : 12554. ScholarBank@NUS Repository. https://doi.org/10.1038/srep12554
Rights: Attribution 4.0 International
Abstract: T cell progenitors are known to arise from the foetal liver in embryos and the bone marrow in adults; however different studies have shown that a pool of T cell progenitors may also exist in the periphery. Here, we identified a lymphoid population resembling peripheral T cell progenitors which transiently seed the epidermis during late embryogenesis in both wild-type and T cell-deficient mice. We named these cells ELCs (Epidermal Lymphoid Cells). ELCs expressed Thy1 and CD2, but lacked CD3 and TCR??/Î 3Î at their surface, reminiscent of the phenotype of extra- or intra- thymic T cell progenitors. Similarly to Dendritic Epidermal T Cells (DETCs), ELCs were radioresistant and capable of self-renewal. However, despite their progenitor-like phenotype and expression of T cell lineage markers within the population, ELCs did not differentiate into conventional T cells or DETCs in in vitro, ex vivo or in vivo differentiation assays. Finally, we show that ELC expressed NK markers and secreted IFN-Î 3 upon stimulation. Therefore we report the discovery of a unique population of lymphoid cells within the murine epidermis that appears related to NK cells with as-yet-unidentified functions.
Source Title: Scientific Reports
URI: https://scholarbank.nus.edu.sg/handle/10635/180450
ISSN: 2045-2322
DOI: 10.1038/srep12554
Rights: Attribution 4.0 International
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