Please use this identifier to cite or link to this item:
Title: Optimization of 3-D hepatocyte culture by controlling the physical and chemical properties of the extra-cellular matrices
Authors: Ng, S.
Wu, Y.-N. 
Yu, H. 
Zhou, Y. 
Toh, Y.-E.
Ho, Z.-Z.
Chia, S.-M. 
Zhu, J.-H. 
Mao, H.-Q. 
Keywords: Cell culture
Confocal microscopy
ECM (extra-cellular matrix)
Issue Date: 2005
Citation: Ng, S., Wu, Y.-N., Yu, H., Zhou, Y., Toh, Y.-E., Ho, Z.-Z., Chia, S.-M., Zhu, J.-H., Mao, H.-Q. (2005). Optimization of 3-D hepatocyte culture by controlling the physical and chemical properties of the extra-cellular matrices. Biomaterials 26 (16) : 3153-3163. ScholarBank@NUS Repository.
Abstract: Hepatocytes are anchorage-dependent cells sensitive to microenvironment; the control of the physicochemical properties of the extra-cellular matrices may be useful to the maintenance of hepatocyte functions in vitro for various applications. In a microcapsule-based 3-D hepatocyte culture microenvironment, we could control the physical properties of the collagen nano-fibres by fine-tuning the complex-coacervation reaction between methylated collagen and terpolymer of hydroxylethyl methacrylate-methyl methacrylate-methylacrylic acid. The physical properties of the nano-fibres were quantitatively characterized using back-scattering confocal microscopy to help optimize the physical support for hepatocyte functions. We further enhanced the chemical properties of the collagen nano-fibres by incorporating galactose onto collagen, which can specifically interact with the asialoglycoprotein receptor on hepatocytes. By correlating a range of collagen nano-fibres of different physicochemical properties with hepatocyte functions, we have identified a specific combination of methylated and galactosylated collagen nano-fibres optimal for maintaining hepatocyte functions in vitro. A model of how the physical and chemical supports interplay to maintain hepatocyte functions is discussed. © 2004 Elsevier Ltd. All rights reserved.
Source Title: Biomaterials
ISSN: 01429612
DOI: 10.1016/j.biomaterials.2004.08.017
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Jul 6, 2022


checked on Jul 6, 2022

Page view(s)

checked on Jun 23, 2022

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.