Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biomaterials.2004.08.017
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dc.titleOptimization of 3-D hepatocyte culture by controlling the physical and chemical properties of the extra-cellular matrices
dc.contributor.authorNg, S.
dc.contributor.authorWu, Y.-N.
dc.contributor.authorYu, H.
dc.contributor.authorZhou, Y.
dc.contributor.authorToh, Y.-E.
dc.contributor.authorHo, Z.-Z.
dc.contributor.authorChia, S.-M.
dc.contributor.authorZhu, J.-H.
dc.contributor.authorMao, H.-Q.
dc.date.accessioned2011-12-07T08:17:00Z
dc.date.available2011-12-07T08:17:00Z
dc.date.issued2005
dc.identifier.citationNg, S., Wu, Y.-N., Yu, H., Zhou, Y., Toh, Y.-E., Ho, Z.-Z., Chia, S.-M., Zhu, J.-H., Mao, H.-Q. (2005). Optimization of 3-D hepatocyte culture by controlling the physical and chemical properties of the extra-cellular matrices. Biomaterials 26 (16) : 3153-3163. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2004.08.017
dc.identifier.issn01429612
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/29734
dc.description.abstractHepatocytes are anchorage-dependent cells sensitive to microenvironment; the control of the physicochemical properties of the extra-cellular matrices may be useful to the maintenance of hepatocyte functions in vitro for various applications. In a microcapsule-based 3-D hepatocyte culture microenvironment, we could control the physical properties of the collagen nano-fibres by fine-tuning the complex-coacervation reaction between methylated collagen and terpolymer of hydroxylethyl methacrylate-methyl methacrylate-methylacrylic acid. The physical properties of the nano-fibres were quantitatively characterized using back-scattering confocal microscopy to help optimize the physical support for hepatocyte functions. We further enhanced the chemical properties of the collagen nano-fibres by incorporating galactose onto collagen, which can specifically interact with the asialoglycoprotein receptor on hepatocytes. By correlating a range of collagen nano-fibres of different physicochemical properties with hepatocyte functions, we have identified a specific combination of methylated and galactosylated collagen nano-fibres optimal for maintaining hepatocyte functions in vitro. A model of how the physical and chemical supports interplay to maintain hepatocyte functions is discussed. © 2004 Elsevier Ltd. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.biomaterials.2004.08.017
dc.sourceScopus
dc.subjectCell culture
dc.subjectCollagen
dc.subjectConfocal microscopy
dc.subjectECM (extra-cellular matrix)
dc.subjectHepatocyte
dc.typeArticle
dc.contributor.departmentPHYSIOLOGY
dc.contributor.departmentORTHOPAEDIC SURGERY
dc.contributor.departmentMATERIALS SCIENCE
dc.description.doi10.1016/j.biomaterials.2004.08.017
dc.description.sourcetitleBiomaterials
dc.description.volume26
dc.description.issue16
dc.description.page3153-3163
dc.description.codenBIMAD
dc.identifier.isiut000226675500037
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