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Title: Optimization of 3-D hepatocyte culture by controlling the physical and chemical properties of the extra-cellular matrices
Authors: Ng, S.
Wu, Y.-N. 
Yu, H. 
Zhou, Y. 
Toh, Y.-E.
Ho, Z.-Z.
Chia, S.-M. 
Zhu, J.-H. 
Mao, H.-Q. 
Keywords: Cell culture
Confocal microscopy
ECM (extra-cellular matrix)
Issue Date: 2005
Citation: Ng, S., Wu, Y.-N., Yu, H., Zhou, Y., Toh, Y.-E., Ho, Z.-Z., Chia, S.-M., Zhu, J.-H., Mao, H.-Q. (2005). Optimization of 3-D hepatocyte culture by controlling the physical and chemical properties of the extra-cellular matrices. Biomaterials 26 (16) : 3153-3163. ScholarBank@NUS Repository.
Abstract: Hepatocytes are anchorage-dependent cells sensitive to microenvironment; the control of the physicochemical properties of the extra-cellular matrices may be useful to the maintenance of hepatocyte functions in vitro for various applications. In a microcapsule-based 3-D hepatocyte culture microenvironment, we could control the physical properties of the collagen nano-fibres by fine-tuning the complex-coacervation reaction between methylated collagen and terpolymer of hydroxylethyl methacrylate-methyl methacrylate-methylacrylic acid. The physical properties of the nano-fibres were quantitatively characterized using back-scattering confocal microscopy to help optimize the physical support for hepatocyte functions. We further enhanced the chemical properties of the collagen nano-fibres by incorporating galactose onto collagen, which can specifically interact with the asialoglycoprotein receptor on hepatocytes. By correlating a range of collagen nano-fibres of different physicochemical properties with hepatocyte functions, we have identified a specific combination of methylated and galactosylated collagen nano-fibres optimal for maintaining hepatocyte functions in vitro. A model of how the physical and chemical supports interplay to maintain hepatocyte functions is discussed. © 2004 Elsevier Ltd. All rights reserved.
Source Title: Biomaterials
ISSN: 01429612
DOI: 10.1016/j.biomaterials.2004.08.017
Appears in Collections:Staff Publications

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