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Title: CD10 marks non-canonical PPAR?-independent adipocyte maturation and browning potential of adipose-derived stem cells
Authors: Chakraborty, Smarajit
Ong, Wee Kiat
Yau, Winifred W. Y. 
Zhou, Zhihong
Bhanu Prakash K.N.
Toh, Sue-Anne 
Han, Weiping
Yen, Paul M. 
Sugii, Shigeki 
Keywords: Adipocytes, beige
Adipocytes, non-canonical activation
Biomarker, adipogenesis
Drug screening, nuclear receptor superfamily
Lipid droplets accumulation
Lipolytic pathways
Neprilysin, NEP, MME
Quality, adipose-derived mesenchymal stem cells
Seahorse analysis, oxidative metabolism
Issue Date: 4-Feb-2021
Publisher: BioMed Central Ltd
Citation: Chakraborty, Smarajit, Ong, Wee Kiat, Yau, Winifred W. Y., Zhou, Zhihong, Bhanu Prakash K.N., Toh, Sue-Anne, Han, Weiping, Yen, Paul M., Sugii, Shigeki (2021-02-04). CD10 marks non-canonical PPAR?-independent adipocyte maturation and browning potential of adipose-derived stem cells. Stem Cell Research and Therapy 12 (1) : 109. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: Background: Effective stem cell therapy is dependent on the stem cell quality that is determined by their differentiation potential, impairment of which leads to poor engraftment and survival into the target cells. However, limitations in our understanding and the lack of reliable markers that can predict their maturation efficacies have hindered the development of stem cells as an effective therapeutic strategy. Our previous study identified CD10, a pro-adipogenic, depot-specific prospective cell surface marker of human adipose-derived stem cells (ASCs). Here, we aim to determine if CD10 can be used as a prospective marker to predict mature adipocyte quality and play a direct role in adipocyte maturation. Methods: We first generated 14 primary human subject-derived ASCs and stable immortalized CD10 knockdown and overexpression lines for 4 subjects by the lentiviral transduction system. To evaluate the role of CD10 in adipogenesis, the adipogenic potential of the human subject samples were scored against their respective CD10 transcript levels. Assessment of UCP1 expression levels was performed to correlate CD10 levels to the browning potential of mature ASCs. Quantitative polymerase chain reaction (qPCR) and Western blot analysis were performed to determine CD10-dependent regulation of various targets. Seahorse analysis of oxidative metabolism and lipolysis assay were studied. Lastly, as a proof-of-concept study, we used CD10 as a prospective marker for screening nuclear receptor ligands library. Results: We identified intrinsic CD10 levels as a positive determinant of adipocyte maturation as well as browning potential of ASCs. Interestingly, CD10 regulates ASC’s adipogenic maturation non-canonically by modulating endogenous lipolysis without affecting the classical peroxisome proliferator-activated receptor gamma (PPAR?)-dependent adipogenic pathways. Furthermore, our CD10-mediated screening analysis identified dexamethasone and retinoic acid as stimulator and inhibitor of adipogenesis, respectively, indicating CD10 as a useful biomarker for pro-adipogenic drug screening. Conclusion: Overall, we establish CD10 as a functionally relevant ASC biomarker, which may be a prerequisite to identify high-quality cell populations for improving metabolic diseases. © 2021, The Author(s).
Source Title: Stem Cell Research and Therapy
ISSN: 1757-6512
DOI: 10.1186/s13287-021-02179-y
Rights: Attribution 4.0 International
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