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Title: A Comparison of retrieved Respiratory Viral Load emitted by infected SARS-CoV-2 patients
Authors: Tham, Kwok Wai 
Coleman, Kristen 
Tay, Douglas 
Ong, Ong
Tan, Kai Sen 
Milton, Don
Koh, ming hui 
Chow, TK 
Chen, Mark
Tambyah, Paul 
Issue Date: 11-Jun-2022
Citation: Tham, Kwok Wai, Coleman, Kristen, Tay, Douglas, Ong, Ong, Tan, Kai Sen, Milton, Don, Koh, ming hui, Chow, TK, Chen, Mark, Tambyah, Paul (2022-06-11). A Comparison of retrieved Respiratory Viral Load emitted by infected SARS-CoV-2 patients. Indoor Air 2022. ScholarBank@NUS Repository.
Abstract: Viral RNA in fine (< 5 m) aerosols from 13 patients infected by the SARS-CoV-2 virus were obtained using the Gesundheit-II (G-II) equipment which collects respiratory emissions. The collection was performed in isolation wards of the National Centre for Infectious Diseases of Singapore under an approved protocol. The patients breathed normally for 30 minutes, talk, and sing for 15 minutes each (with 30 minutes rest in between activity) into a specially designed aerosol collector in two size fractions. The coarse fraction (> 5 m) and the fine aerosols (< 5 m) are subsequently collected and subjected to PCR analysis for their viral load quantification. Viral RNA detected from 59% of the patients showed that patients earlier in illness were more likely to emit detectable RNA, and loads differed significantly between breathing, talking, and singing.
Source Title: Indoor Air 2022
Appears in Collections:Staff Publications

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