Please use this identifier to cite or link to this item: https://doi.org/10.3390/microorganisms9061193
Title: Comparative Transcriptomic and Molecular Pathway Analyses of HL-CZ Human Pro-Monocytic Cells Expressing SARS-CoV-2 Spike S1, S2, NP, NSP15 and NSP16 Genes
Authors: Sharma, Anshika
Ong, Joe W 
Loke, Mun Fai 
Chua, Eng Guan
Lee, Joseph J
Choi, Hyung Won 
Tan, Yee Joo 
Lal, Sunil K
Chow, Vincent T 
Keywords: Science & Technology
Life Sciences & Biomedicine
Microbiology
SARS-CoV-2
RNA-sequencing
Spike S1
Spike S2
Nucleocapsid (NP)
endoribonuclease (NSP15)
Methyltransferase (NSP16)
ENDOPLASMIC-RETICULUM STRESS
SARS CORONAVIRUS INFECTION
NUCLEOCAPSID PROTEIN
UNFOLDED PROTEIN
IMMUNE-RESPONSES
APOPTOSIS
DISEASE
MODULATION
REVEALS
PACKAGE
Issue Date: 1-Jun-2021
Publisher: MDPI
Citation: Sharma, Anshika, Ong, Joe W, Loke, Mun Fai, Chua, Eng Guan, Lee, Joseph J, Choi, Hyung Won, Tan, Yee Joo, Lal, Sunil K, Chow, Vincent T (2021-06-01). Comparative Transcriptomic and Molecular Pathway Analyses of HL-CZ Human Pro-Monocytic Cells Expressing SARS-CoV-2 Spike S1, S2, NP, NSP15 and NSP16 Genes. MICROORGANISMS 9 (6). ScholarBank@NUS Repository. https://doi.org/10.3390/microorganisms9061193
Abstract: The ongoing COVID-19 pandemic is a clear and present threat to global public health. Research into how the causative SARS-CoV-2 virus together with its individual constituent genes and proteins interact with target host cells can facilitate the development of improved strategies to manage the acute and long-term complications of COVID-19. In this study, to better understand the biological roles of critical SARS-CoV-2 proteins, we determined and compared the host tran-scriptomic responses of the HL-CZ human pro-monocytic cell line upon transfection with key viral genes encoding the spike S1 subunit, S2 subunit, nucleocapsid protein (NP), NSP15 (endoribonu-clease), and NSP16 (2′-O-ribose-methyltransferase). RNA sequencing followed by gene set enrich-ment analysis and other bioinformatics tools revealed that host genes associated with topologically incorrect protein, virus receptor activity, heat shock protein binding, endoplasmic reticulum stress, antigen processing and presentation were up-regulated in the presence of viral spike S1 expression. With spike S2 expression, pro-monocytic genes associated with the interferon-gamma-mediated signaling pathway, regulation of phosphatidylinositol 3-kinase activity, adipocytokine signaling pathway, and insulin signaling pathway were down-regulated, whereas those associated with cy-tokine-mediated signaling were up-regulated. The expression of NSP15 induced the up-regulation of genes associated with neutrophil degranulation, neutrophil-mediated immunity, oxidative phos-phorylation, prion disease, and pathways of neurodegeneration. The expression of NSP16 resulted in the down-regulation of genes associated with S-adenosylmethionine-dependent methyltransfer-ase activity. The expression of NP down-regulated genes associated with positive regulation of neu-rogenesis, nervous system development, and heart development. Taken together, the complex tran-scriptomic alterations arising from these viral-host gene interactions offer useful insights into host genes and their pathways that potentially contribute to SARS-CoV-2 pathogenesis.
Source Title: MICROORGANISMS
URI: https://scholarbank.nus.edu.sg/handle/10635/218829
ISSN: 20762607
DOI: 10.3390/microorganisms9061193
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