Please use this identifier to cite or link to this item: https://doi.org/10.1186/s12885-018-4840-5
Title: Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines 11 Medical and Health Sciences 1107 Immunology
Authors: Tan, K.-T 
Ding, L.-W 
Sun, Q.-Y 
Lao, Z.-T 
Chien, W 
Ren, X
Xiao, J.-F 
Loh, X.Y 
Xu, L 
Lill, M
Mayakonda, A 
Lin, D.-C
Yang, H 
Koeffler, H.P 
Keywords: B lymphocyte receptor
immunoglobulin
T lymphocyte receptor
lymphocyte antigen receptor
RNA
Article
B lymphocyte
B-lymphocyte cell line
blood cancer cell line
cancer classification
cell infiltration
cell population
clonal evolution
clonal variation
cohort analysis
Epstein Barr virus
gastric cancer cell line
gene
gene rearrangement
genetic analysis
hematopoietic tissue
human
human cell
immunoglobulin gene
KE 97 cell line
lymphocyte subpopulation
lymphoid cell line
lymphoid tissue
RNA sequence
solid malignant neoplasm
stroma cell
TCR gene
cytology
genetics
hematologic disease
lymphocyte
neoplasm
tumor cell line
B-Lymphocytes
Cell Line, Tumor
Hematologic Neoplasms
Herpesvirus 4, Human
Humans
Lymphocytes
Neoplasms
Receptors, Antigen, B-Cell
Receptors, Antigen, T-Cell
RNA
Issue Date: 2018
Citation: Tan, K.-T, Ding, L.-W, Sun, Q.-Y, Lao, Z.-T, Chien, W, Ren, X, Xiao, J.-F, Loh, X.Y, Xu, L, Lill, M, Mayakonda, A, Lin, D.-C, Yang, H, Koeffler, H.P (2018). Profiling the B/T cell receptor repertoire of lymphocyte derived cell lines 11 Medical and Health Sciences 1107 Immunology. BMC Cancer 18 (1) : 940. ScholarBank@NUS Repository. https://doi.org/10.1186/s12885-018-4840-5
Abstract: Background: Clonal VDJ rearrangement of B/T cell receptors (B/TCRs) occurring during B/T lymphocyte development has been used as a marker to track the clonality of B/T cell populations. Methods: We systematically profiled the B/T cell receptor repertoire of 936 cancer cell lines across a variety of cancer types as well as 462 Epstein-Barr Virus (EBV) transformed normal B lymphocyte lines using RNA sequencing data. Results: Rearranged B/TCRs were readily detected in cell lines derived from lymphocytes, and subclonality or potential biclonality were found in a number of blood cancer cell lines. Clonal BCR/TCR rearrangements were detected in several blast phase CML lines and unexpectedly, one gastric cancer cell line (KE-97), reflecting a lymphoid origin of these cells. Notably, clonality was highly prevalent in EBV transformed B lymphocytes, suggesting either transformation only occurred in a few B cells or those with a growth advantage dominated the transformed population through clonal evolution. Conclusions: Our analysis reveals the complexity and heterogeneity of the BCR/TCR rearrangement repertoire and provides a unique insight into the clonality of lymphocyte derived cell lines. © 2018 The Author(s).
Source Title: BMC Cancer
URI: https://scholarbank.nus.edu.sg/handle/10635/175363
ISSN: 1471-2407
DOI: 10.1186/s12885-018-4840-5
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