Please use this identifier to cite or link to this item: https://doi.org/10.14814/phy2.13437
Title: Assessing the effects of mitofusin 2 deficiency in the adult heart using 3D electron tomography
Authors: Kalkhoran, S.B
Hall, A.R
White, I.J
Cooper, J
Fan, Q 
Ong, S.-B 
Hernández-Reséndiz, S 
Cabrera-Fuentes, H 
Chinda, K
Chakraborty, B 
Dorn, G.W
Yellon, D.M
Hausenloy, D.J 
Keywords: mitofusin 2
guanosine triphosphatase
Mfn2 protein, mouse
adult
animal experiment
animal tissue
anisotropy
Article
cell structure
cellular parameters
controlled study
electron microscope
electron tomography
female
heart
heart mitochondrion
image processing
mitochondrial dynamics
mitochondrial elongation
mitochondrial flatness
mitochondrial roundness
mitochondrial volume density
mouse
nonhuman
polymerase chain reaction
protein deficiency
staining
three dimensional imaging
transmission electron microscopy
two-dimensional imaging
animal
cardiac muscle cell
deficiency
electron tomography
genetics
metabolism
muscle mitochondrion
procedures
sarcoplasmic reticulum
three dimensional imaging
ultrastructure
Animals
Electron Microscope Tomography
GTP Phosphohydrolases
Imaging, Three-Dimensional
Mice
Mitochondria, Muscle
Myocytes, Cardiac
Sarcoplasmic Reticulum
Issue Date: 2017
Citation: Kalkhoran, S.B, Hall, A.R, White, I.J, Cooper, J, Fan, Q, Ong, S.-B, Hernández-Reséndiz, S, Cabrera-Fuentes, H, Chinda, K, Chakraborty, B, Dorn, G.W, Yellon, D.M, Hausenloy, D.J (2017). Assessing the effects of mitofusin 2 deficiency in the adult heart using 3D electron tomography. Physiological Reports 5 (17) : e13437. ScholarBank@NUS Repository. https://doi.org/10.14814/phy2.13437
Abstract: The effects of mitofusin 2 (MFN2) deficiency, on mitochondrial morphology and the mitochondria-junctional sarcoplasmic reticulum (jSR) complex in the adult heart, have been previously investigated using 2D electron microscopy, an approach which is unable to provide a 3D spatial assessment of these imaging parameters. Here, we use 3D electron tomography to show that MFN2-deficient mitochondria are larger in volume, more elongated, and less rounded; have fewer mitochondria-jSR contacts, and an increase in the distance between mitochondria and jSR, when compared to WT mitochondria. In comparison to 2D electron microscopy, 3D electron tomography can provide further insights into mitochondrial morphology and the mitochondria- jSR complex in the adult heart. © 2017 The Authors.
Source Title: Physiological Reports
URI: https://scholarbank.nus.edu.sg/handle/10635/175200
ISSN: 2051817X
DOI: 10.14814/phy2.13437
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