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https://doi.org/10.14814/phy2.13437
| Title: | Assessing the effects of mitofusin 2 deficiency in the adult heart using 3D electron tomography | Authors: | Kalkhoran, S.B Hall, A.R White, I.J Cooper, J Fan, Q Ong, S.-B Hernández-Reséndiz, S Cabrera-Fuentes, H Chinda, K Chakraborty, B Dorn, G.W Yellon, D.M Hausenloy, D.J |
Keywords: | mitofusin 2 guanosine triphosphatase Mfn2 protein, mouse adult animal experiment animal tissue anisotropy Article cell structure cellular parameters controlled study electron microscope electron tomography female heart heart mitochondrion image processing mitochondrial dynamics mitochondrial elongation mitochondrial flatness mitochondrial roundness mitochondrial volume density mouse nonhuman polymerase chain reaction protein deficiency staining three dimensional imaging transmission electron microscopy two-dimensional imaging animal cardiac muscle cell deficiency electron tomography genetics metabolism muscle mitochondrion procedures sarcoplasmic reticulum three dimensional imaging ultrastructure Animals Electron Microscope Tomography GTP Phosphohydrolases Imaging, Three-Dimensional Mice Mitochondria, Muscle Myocytes, Cardiac Sarcoplasmic Reticulum |
Issue Date: | 2017 | Citation: | Kalkhoran, S.B, Hall, A.R, White, I.J, Cooper, J, Fan, Q, Ong, S.-B, Hernández-Reséndiz, S, Cabrera-Fuentes, H, Chinda, K, Chakraborty, B, Dorn, G.W, Yellon, D.M, Hausenloy, D.J (2017). Assessing the effects of mitofusin 2 deficiency in the adult heart using 3D electron tomography. Physiological Reports 5 (17) : e13437. ScholarBank@NUS Repository. https://doi.org/10.14814/phy2.13437 | Abstract: | The effects of mitofusin 2 (MFN2) deficiency, on mitochondrial morphology and the mitochondria-junctional sarcoplasmic reticulum (jSR) complex in the adult heart, have been previously investigated using 2D electron microscopy, an approach which is unable to provide a 3D spatial assessment of these imaging parameters. Here, we use 3D electron tomography to show that MFN2-deficient mitochondria are larger in volume, more elongated, and less rounded; have fewer mitochondria-jSR contacts, and an increase in the distance between mitochondria and jSR, when compared to WT mitochondria. In comparison to 2D electron microscopy, 3D electron tomography can provide further insights into mitochondrial morphology and the mitochondria- jSR complex in the adult heart. © 2017 The Authors. | Source Title: | Physiological Reports | URI: | https://scholarbank.nus.edu.sg/handle/10635/175200 | ISSN: | 2051817X | DOI: | 10.14814/phy2.13437 |
| Appears in Collections: | Elements Staff Publications |
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