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https://doi.org/10.1038/srep03232
Title: | Defining the expression hierarchy of latent T-cell epitopes in Epstein-Barr virus infection with TCR-like antibodies | Authors: | Sim A.C.N. Too C.T. Oo M.Z. Lai J. Eio M.Y. Song Z. Srinivasan N. Tan D.A.L. Pang S.W. Gan S.U. Lee K.O. Loh T.K.S. Chen J. Chan S.H. Macary P.A. |
Keywords: | EBV-associated membrane antigen, Epstein-Barr virus EBV-encoded nuclear antigen 1 epitope Epstein Barr virus antigen HLA A2 antigen HLA-A*02:01 antigen lymphocyte antigen receptor matrix protein monoclonal antibody virus antigen animal Bagg albino mouse CD8+ T lymphocyte Epstein Barr virus Epstein Barr virus infection human immunology mouse nonobese diabetic mouse SCID mouse tumor cell line virus latency Animals Antibodies, Monoclonal Antigens, Viral CD8-Positive T-Lymphocytes Cell Line, Tumor Epitopes, T-Lymphocyte Epstein-Barr Virus Infections Epstein-Barr Virus Nuclear Antigens Herpesvirus 4, Human HLA-A2 Antigen Humans Mice Mice, Inbred BALB C Mice, Inbred NOD Mice, SCID Receptors, Antigen, T-Cell Viral Matrix Proteins Virus Latency |
Issue Date: | 2013 | Citation: | Sim A.C.N., Too C.T., Oo M.Z., Lai J., Eio M.Y., Song Z., Srinivasan N., Tan D.A.L., Pang S.W., Gan S.U., Lee K.O., Loh T.K.S., Chen J., Chan S.H., Macary P.A. (2013). Defining the expression hierarchy of latent T-cell epitopes in Epstein-Barr virus infection with TCR-like antibodies. Scientific Reports 3 : 3232. ScholarBank@NUS Repository. https://doi.org/10.1038/srep03232 | Abstract: | Epstein-Barr virus (EBV) is a gamma herpesvirus that causes a life-long latent infection in human hosts. The latent gene products LMP1, LMP2A and EBNA1 are expressed by EBV-associated tumors and peptide epitopes derived from these can be targeted by CD8 Cytotoxic T-Lymphocyte (CTL) lines. Whilst CTL-based methodologies can be utilized to infer the presence of specific latent epitopes, they do not allow a direct visualization or quantitation of these epitopes. Here, we describe the characterization of three TCR-like monoclonal antibodies (mAbs) targeting the latent epitopes LMP1 125-133, LMP2A 426-434 or EBNA1 562-570 in association with HLA-A0201. These are employed to map the expression hierarchy of endogenously generated EBV epitopes. The dominance of EBNA1 562-570 in association with HLA-A0201 was consistently observed in cell lines and EBV-associated tumor biopsies. These data highlight the discordance between MHC-epitope density and frequencies of associated CTL with implications for cell-based immunotherapies and/or vaccines for EBV-associated disease. | Source Title: | Scientific Reports | URI: | https://scholarbank.nus.edu.sg/handle/10635/174586 | ISSN: | 2045-2322 | DOI: | 10.1038/srep03232 |
Appears in Collections: | Staff Publications Elements |
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