Please use this identifier to cite or link to this item: https://doi.org/10.1038/srep03232
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dc.titleDefining the expression hierarchy of latent T-cell epitopes in Epstein-Barr virus infection with TCR-like antibodies
dc.contributor.authorSim A.C.N.
dc.contributor.authorToo C.T.
dc.contributor.authorOo M.Z.
dc.contributor.authorLai J.
dc.contributor.authorEio M.Y.
dc.contributor.authorSong Z.
dc.contributor.authorSrinivasan N.
dc.contributor.authorTan D.A.L.
dc.contributor.authorPang S.W.
dc.contributor.authorGan S.U.
dc.contributor.authorLee K.O.
dc.contributor.authorLoh T.K.S.
dc.contributor.authorChen J.
dc.contributor.authorChan S.H.
dc.contributor.authorMacary P.A.
dc.date.accessioned2020-09-08T03:21:10Z
dc.date.available2020-09-08T03:21:10Z
dc.date.issued2013
dc.identifier.citationSim A.C.N., Too C.T., Oo M.Z., Lai J., Eio M.Y., Song Z., Srinivasan N., Tan D.A.L., Pang S.W., Gan S.U., Lee K.O., Loh T.K.S., Chen J., Chan S.H., Macary P.A. (2013). Defining the expression hierarchy of latent T-cell epitopes in Epstein-Barr virus infection with TCR-like antibodies. Scientific Reports 3 : 3232. ScholarBank@NUS Repository. https://doi.org/10.1038/srep03232
dc.identifier.issn2045-2322
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/174586
dc.description.abstractEpstein-Barr virus (EBV) is a gamma herpesvirus that causes a life-long latent infection in human hosts. The latent gene products LMP1, LMP2A and EBNA1 are expressed by EBV-associated tumors and peptide epitopes derived from these can be targeted by CD8 Cytotoxic T-Lymphocyte (CTL) lines. Whilst CTL-based methodologies can be utilized to infer the presence of specific latent epitopes, they do not allow a direct visualization or quantitation of these epitopes. Here, we describe the characterization of three TCR-like monoclonal antibodies (mAbs) targeting the latent epitopes LMP1 125-133, LMP2A 426-434 or EBNA1 562-570 in association with HLA-A0201. These are employed to map the expression hierarchy of endogenously generated EBV epitopes. The dominance of EBNA1 562-570 in association with HLA-A0201 was consistently observed in cell lines and EBV-associated tumor biopsies. These data highlight the discordance between MHC-epitope density and frequencies of associated CTL with implications for cell-based immunotherapies and/or vaccines for EBV-associated disease.
dc.sourceUnpaywall 20200831
dc.subjectEBV-associated membrane antigen, Epstein-Barr virus
dc.subjectEBV-encoded nuclear antigen 1
dc.subjectepitope
dc.subjectEpstein Barr virus antigen
dc.subjectHLA A2 antigen
dc.subjectHLA-A*02:01 antigen
dc.subjectlymphocyte antigen receptor
dc.subjectmatrix protein
dc.subjectmonoclonal antibody
dc.subjectvirus antigen
dc.subjectanimal
dc.subjectBagg albino mouse
dc.subjectCD8+ T lymphocyte
dc.subjectEpstein Barr virus
dc.subjectEpstein Barr virus infection
dc.subjecthuman
dc.subjectimmunology
dc.subjectmouse
dc.subjectnonobese diabetic mouse
dc.subjectSCID mouse
dc.subjecttumor cell line
dc.subjectvirus latency
dc.subjectAnimals
dc.subjectAntibodies, Monoclonal
dc.subjectAntigens, Viral
dc.subjectCD8-Positive T-Lymphocytes
dc.subjectCell Line, Tumor
dc.subjectEpitopes, T-Lymphocyte
dc.subjectEpstein-Barr Virus Infections
dc.subjectEpstein-Barr Virus Nuclear Antigens
dc.subjectHerpesvirus 4, Human
dc.subjectHLA-A2 Antigen
dc.subjectHumans
dc.subjectMice
dc.subjectMice, Inbred BALB C
dc.subjectMice, Inbred NOD
dc.subjectMice, SCID
dc.subjectReceptors, Antigen, T-Cell
dc.subjectViral Matrix Proteins
dc.subjectVirus Latency
dc.typeArticle
dc.contributor.departmentDEPT OF BIOLOGICAL SCIENCES
dc.contributor.departmentDEPT OF MEDICINE
dc.contributor.departmentDEPT OF MICROBIOLOGY & IMMUNOLOGY
dc.contributor.departmentDEPT OF OTOLARYNGOLOGY
dc.contributor.departmentDEPT OF SURGERY
dc.description.doi10.1038/srep03232
dc.description.sourcetitleScientific Reports
dc.description.volume3
dc.description.page3232
dc.published.statePublished
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