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https://doi.org/10.1038/s41467-017-02030-0
Title: | Non-canonical activation of OmpR drives acid and osmotic stress responses in single bacterial cells | Authors: | Chakraborty S. Winardhi R.S. Morgan L.K. Yan J. Kenney L.J. |
Keywords: | benzoic acid outer membrane protein regulator phosphotransferase phosphotransferase envz unclassified drug acid bacterial protein osmolarity response regulator proteins transactivator protein acid activation acidification bacterium cells and cell components cytoplasm enzyme activity eukaryote organic acid osmosis pH acidification Article atomic force microscopy bacterial cell cell activation cell pH cytoplasm decarboxylation dimerization DNA binding enzyme phosphorylation Escherichia coli nonhuman osmolality osmotic stress plasmid protein DNA interaction Salmonella enterica Salmonella enterica serovar Typhimurium single cell analysis chemistry cytology gene expression profiling gene expression regulation genetics metabolism osmoregulation osmotic pressure pH procedures single cell analysis Bacteria (microorganisms) Eukaryota Salmonella Acids Bacterial Proteins Cytoplasm Escherichia coli Gene Expression Profiling Gene Expression Regulation, Bacterial Hydrogen-Ion Concentration Osmoregulation Osmotic Pressure Salmonella typhimurium Single-Cell Analysis Trans-Activators |
Issue Date: | 2017 | Publisher: | Nature Publishing Group | Citation: | Chakraborty S., Winardhi R.S., Morgan L.K., Yan J., Kenney L.J. (2017). Non-canonical activation of OmpR drives acid and osmotic stress responses in single bacterial cells. Nature Communications 8 (1) : 1587. ScholarBank@NUS Repository. https://doi.org/10.1038/s41467-017-02030-0 | Abstract: | Unlike eukaryotes, bacteria undergo large changes in osmolality and cytoplasmic pH. It has been described that during acid stress, bacteria internal pH promptly acidifies, followed by recovery. Here, using pH imaging in single living cells, we show that following acid stress, bacteria maintain an acidic cytoplasm and the osmotic stress transcription factor OmpR is required for acidification. The activation of this response is non-canonical, involving a regulatory mechanism requiring the OmpR cognate kinase EnvZ, but not OmpR phosphorylation. Single cell analysis further identifies an intracellular pH threshold ~6.5. Acid stress reduces the internal pH below this threshold, increasing OmpR dimerization and DNA binding. During osmotic stress, the internal pH is above the threshold, triggering distinct OmpR-related pathways. Preventing intracellular acidification of Salmonella renders it avirulent, suggesting that acid stress pathways represent a potential therapeutic target. These results further emphasize the advantages of single cell analysis over studies of population averages. © 2017 The Author(s). | Source Title: | Nature Communications | URI: | https://scholarbank.nus.edu.sg/handle/10635/174484 | ISSN: | 2041-1723 | DOI: | 10.1038/s41467-017-02030-0 |
Appears in Collections: | Elements Staff Publications |
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