Please use this identifier to cite or link to this item: https://doi.org/10.1038/s41467-017-02030-0
Title: Non-canonical activation of OmpR drives acid and osmotic stress responses in single bacterial cells
Authors: Chakraborty S. 
Winardhi R.S. 
Morgan L.K.
Yan J. 
Kenney L.J. 
Keywords: benzoic acid
outer membrane protein regulator
phosphotransferase
phosphotransferase envz
unclassified drug
acid
bacterial protein
osmolarity response regulator proteins
transactivator protein
acid activation
acidification
bacterium
cells and cell components
cytoplasm
enzyme activity
eukaryote
organic acid
osmosis
pH
acidification
Article
atomic force microscopy
bacterial cell
cell activation
cell pH
cytoplasm
decarboxylation
dimerization
DNA binding
enzyme phosphorylation
Escherichia coli
nonhuman
osmolality
osmotic stress
plasmid
protein DNA interaction
Salmonella enterica
Salmonella enterica serovar Typhimurium
single cell analysis
chemistry
cytology
gene expression profiling
gene expression regulation
genetics
metabolism
osmoregulation
osmotic pressure
pH
procedures
single cell analysis
Bacteria (microorganisms)
Eukaryota
Salmonella
Acids
Bacterial Proteins
Cytoplasm
Escherichia coli
Gene Expression Profiling
Gene Expression Regulation, Bacterial
Hydrogen-Ion Concentration
Osmoregulation
Osmotic Pressure
Salmonella typhimurium
Single-Cell Analysis
Trans-Activators
Issue Date: 2017
Publisher: Nature Publishing Group
Citation: Chakraborty S., Winardhi R.S., Morgan L.K., Yan J., Kenney L.J. (2017). Non-canonical activation of OmpR drives acid and osmotic stress responses in single bacterial cells. Nature Communications 8 (1) : 1587. ScholarBank@NUS Repository. https://doi.org/10.1038/s41467-017-02030-0
Abstract: Unlike eukaryotes, bacteria undergo large changes in osmolality and cytoplasmic pH. It has been described that during acid stress, bacteria internal pH promptly acidifies, followed by recovery. Here, using pH imaging in single living cells, we show that following acid stress, bacteria maintain an acidic cytoplasm and the osmotic stress transcription factor OmpR is required for acidification. The activation of this response is non-canonical, involving a regulatory mechanism requiring the OmpR cognate kinase EnvZ, but not OmpR phosphorylation. Single cell analysis further identifies an intracellular pH threshold ~6.5. Acid stress reduces the internal pH below this threshold, increasing OmpR dimerization and DNA binding. During osmotic stress, the internal pH is above the threshold, triggering distinct OmpR-related pathways. Preventing intracellular acidification of Salmonella renders it avirulent, suggesting that acid stress pathways represent a potential therapeutic target. These results further emphasize the advantages of single cell analysis over studies of population averages. © 2017 The Author(s).
Source Title: Nature Communications
URI: https://scholarbank.nus.edu.sg/handle/10635/174484
ISSN: 2041-1723
DOI: 10.1038/s41467-017-02030-0
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