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Title: Enterovirus 71 infection of motor neuron-like NSC-34 cells undergoes a non-lytic exit pathway
Authors: Too, I.H.K
Yeo, H 
Sessions, O.M 
Yan, B
Libau, E.A 
Howe, J.L.C
Lim, Z.Q
Suku-Maran, S
Ong, W.-Y 
Chua, K.B
Wong, B.S 
Chow, V.T.K 
Alonso, S 
Keywords: autophagy
cell line
Enterovirus A
virus culture
virus entry
virus release
virus replication
Cell Line
Enterovirus A, Human
Motor Neurons
Virus Cultivation
Virus Internalization
Virus Release
Virus Replication
Issue Date: 2016
Citation: Too, I.H.K, Yeo, H, Sessions, O.M, Yan, B, Libau, E.A, Howe, J.L.C, Lim, Z.Q, Suku-Maran, S, Ong, W.-Y, Chua, K.B, Wong, B.S, Chow, V.T.K, Alonso, S (2016). Enterovirus 71 infection of motor neuron-like NSC-34 cells undergoes a non-lytic exit pathway. Scientific Reports 6 : 36983. ScholarBank@NUS Repository.
Abstract: Enterovirus 71 (EV71) causing Hand, Foot and Mouth Disease, is regarded as the most important neurotropic virus worldwide. EV71 is believed to replicate in muscles and infect motor neurons to reach the central nervous system (CNS). To further investigate the mechanisms involved, we have employed the motor neuron cell line NSC-34. NSC-34 cells were permissive to EV71 and virus production yields were strain-dependent with differential efficacy at the entry, replication and egress steps. Furthermore, unlike all the other cell lines previously reported, EV71-infected NSC-34 cells neither displayed cytopathic effect nor underwent apoptosis. Instead, autophagy was markedly up-regulated and virus-containing autophagic vacuoles were isolated from the culture supernatant, providing the first experimental evidence that EV71 can adopt a non-lytic exit pathway. Finally, the ability of EV71 to infect productively NSC-34 cells correlated with its ability to invade the CNS in vivo, supporting the relevance of NSC-34 cells to study the intrinsic neurovirulence of EV71 strains. © 2016 The Author(s).
Source Title: Scientific Reports
ISSN: 20452322
DOI: 10.1038/srep36983
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