Please use this identifier to cite or link to this item: https://doi.org/10.1021/acs.analchem.6b02073
Title: Specific Light-Up Probe with Aggregation-Induced Emission for Facile Detection of Chymase
Authors: Zhang, Ruoyu 
Zhang, Chong-Jing 
Feng, Guangxue 
Hu, Fang 
Wang, Jigang
Liu, Bin 
Keywords: Science & Technology
Physical Sciences
Chemistry, Analytical
Chemistry
GUIDED PHOTODYNAMIC ABLATION
ON FLUORESCENT-PROBE
CANCER-CELLS
MAST-CELL
IN-SITU
THERAPEUTIC RESPONSES
TETRAPHENYLETHENE
BIOPROBE
INFLAMMATION
APOPTOSIS
Issue Date: 20-Sep-2016
Publisher: AMER CHEMICAL SOC
Citation: Zhang, Ruoyu, Zhang, Chong-Jing, Feng, Guangxue, Hu, Fang, Wang, Jigang, Liu, Bin (2016-09-20). Specific Light-Up Probe with Aggregation-Induced Emission for Facile Detection of Chymase. ANALYTICAL CHEMISTRY 88 (18) : 9111-9117. ScholarBank@NUS Repository. https://doi.org/10.1021/acs.analchem.6b02073
Abstract: © 2016 American Chemical Society. Human chymases are important proteases abundant in mast cell granules. The elevated level of chymases and other serine proteases is closely related to inflammatory and immunoregulatory functions. Monitoring of the chymase level is very important, however, the existing methods remain limited and insufficient. In this work, a light-up probe of TPETH-2(CFTERD3) (where CFTERD is Cys-Phe-Thr-Glu-Arg-Asp) was developed for chymase detection. The probe has low fluorescent signal in aqueous media, but its solubility can be changed after hydrolysis by chymase, giving significant fluorescence turn-on with a high signal-to-noise (S/N) ratio. The probe has excellent selectivity to chymase compared to other proteins and can effectively differentiate chymase from other enzymes (e.g., chymotrypsin and trypsin) in the same family (E.C. 3.4.21). The detection limit is calculated to be 0.1 ng/mL in PBS buffer with a linear range of 0-9.0 ng/mL. A comparison study using TPETH-2(CFTERD2) as the probe reveals the importance of molecular design in realizing the high S/N ratio. TPETH-2(CFTERD3) thus represents a simple turn-on probe for chymase detection, with real-time and direct readout and also excellent sensitivity and selectivity.
Source Title: ANALYTICAL CHEMISTRY
URI: https://scholarbank.nus.edu.sg/handle/10635/169775
ISSN: 00032700
15206882
DOI: 10.1021/acs.analchem.6b02073
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