Please use this identifier to cite or link to this item: https://doi.org/10.1021/acs.analchem.6b02073
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dc.titleSpecific Light-Up Probe with Aggregation-Induced Emission for Facile Detection of Chymase
dc.contributor.authorZhang, Ruoyu
dc.contributor.authorZhang, Chong-Jing
dc.contributor.authorFeng, Guangxue
dc.contributor.authorHu, Fang
dc.contributor.authorWang, Jigang
dc.contributor.authorLiu, Bin
dc.date.accessioned2020-06-15T06:47:56Z
dc.date.available2020-06-15T06:47:56Z
dc.date.issued2016-09-20
dc.identifier.citationZhang, Ruoyu, Zhang, Chong-Jing, Feng, Guangxue, Hu, Fang, Wang, Jigang, Liu, Bin (2016-09-20). Specific Light-Up Probe with Aggregation-Induced Emission for Facile Detection of Chymase. ANALYTICAL CHEMISTRY 88 (18) : 9111-9117. ScholarBank@NUS Repository. https://doi.org/10.1021/acs.analchem.6b02073
dc.identifier.issn00032700
dc.identifier.issn15206882
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/169775
dc.description.abstract© 2016 American Chemical Society. Human chymases are important proteases abundant in mast cell granules. The elevated level of chymases and other serine proteases is closely related to inflammatory and immunoregulatory functions. Monitoring of the chymase level is very important, however, the existing methods remain limited and insufficient. In this work, a light-up probe of TPETH-2(CFTERD3) (where CFTERD is Cys-Phe-Thr-Glu-Arg-Asp) was developed for chymase detection. The probe has low fluorescent signal in aqueous media, but its solubility can be changed after hydrolysis by chymase, giving significant fluorescence turn-on with a high signal-to-noise (S/N) ratio. The probe has excellent selectivity to chymase compared to other proteins and can effectively differentiate chymase from other enzymes (e.g., chymotrypsin and trypsin) in the same family (E.C. 3.4.21). The detection limit is calculated to be 0.1 ng/mL in PBS buffer with a linear range of 0-9.0 ng/mL. A comparison study using TPETH-2(CFTERD2) as the probe reveals the importance of molecular design in realizing the high S/N ratio. TPETH-2(CFTERD3) thus represents a simple turn-on probe for chymase detection, with real-time and direct readout and also excellent sensitivity and selectivity.
dc.language.isoen
dc.publisherAMER CHEMICAL SOC
dc.sourceElements
dc.subjectScience & Technology
dc.subjectPhysical Sciences
dc.subjectChemistry, Analytical
dc.subjectChemistry
dc.subjectGUIDED PHOTODYNAMIC ABLATION
dc.subjectON FLUORESCENT-PROBE
dc.subjectCANCER-CELLS
dc.subjectMAST-CELL
dc.subjectIN-SITU
dc.subjectTHERAPEUTIC RESPONSES
dc.subjectTETRAPHENYLETHENE
dc.subjectBIOPROBE
dc.subjectINFLAMMATION
dc.subjectAPOPTOSIS
dc.typeArticle
dc.date.updated2020-06-10T10:41:34Z
dc.contributor.departmentCHEMICAL & BIOMOLECULAR ENGINEERING
dc.description.doi10.1021/acs.analchem.6b02073
dc.description.sourcetitleANALYTICAL CHEMISTRY
dc.description.volume88
dc.description.issue18
dc.description.page9111-9117
dc.published.statePublished
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