Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0179642
Title: Subcellular electrical stimulation of neurons enhances the myelination of axons by oligodendrocytes
Authors: Lee H.U. 
Blasiak A. 
Agrawal D.R.
Loong D.T.B. 
Thakor N.V. 
All A.H. 
Ho J.S. 
Yang I.H. 
Keywords: animal cell
animal tissue
Article
axon
cell body
cell differentiation
cell isolation
controlled study
electric field
electrostimulation
embryo
limit of quantitation
microfluidics
mouse
myelination
nerve cell
nonhuman
oligodendroglia
rat
simulation
animal
axon
cell fractionation
nerve cell
oligodendroglia
physiology
Animals
Axons
Electric Stimulation
Microfluidics
Neurons
Oligodendroglia
Subcellular Fractions
Issue Date: 2017
Publisher: Public Library of Science
Citation: Lee H.U., Blasiak A., Agrawal D.R., Loong D.T.B., Thakor N.V., All A.H., Ho J.S., Yang I.H. (2017). Subcellular electrical stimulation of neurons enhances the myelination of axons by oligodendrocytes. PLoS ONE 12 (7) : e0179642. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0179642
Abstract: Myelin formation has been identified as a modulator of neural plasticity. New tools are required to investigate the mechanisms by which environmental inputs and neural activity regulate myelination patterns. In this study, we demonstrate a microfluidic compartmentalized culture system with integrated electrical stimulation capabilities that can induce neural activity by whole cell and focal stimulation. A set of electric field simulations was performed to confirm spatial restriction of the electrical input in the compartmentalized culture system. We further demonstrate that electrode localization is a key consideration for generating uniform the stimulation of neuron and oligodendrocytes within the compartments. Using three configurations of the electrodes we tested the effects of subcellular activation of neural activity on distal axon myelination with oligodendrocytes. We further investigated if oligodendrocytes have to be exposed to the electrical field to induce axon myelination. An isolated stimulation of cell bodies and proximal axons had the same effect as an isolated stimulation of distal axons co-cultured with oligodendrocytes, and the two modes had a non-different result than whole cell stimulation. Our platform enabled the demonstration that electrical stimulation enhances oligodendrocyte maturation and myelin formation independent of the input localization and oligodendrocyte exposure to the electrical field. © 2017 Lee et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Source Title: PLoS ONE
URI: https://scholarbank.nus.edu.sg/handle/10635/165789
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0179642
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