Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pone.0179642
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dc.titleSubcellular electrical stimulation of neurons enhances the myelination of axons by oligodendrocytes
dc.contributor.authorLee H.U.
dc.contributor.authorBlasiak A.
dc.contributor.authorAgrawal D.R.
dc.contributor.authorLoong D.T.B.
dc.contributor.authorThakor N.V.
dc.contributor.authorAll A.H.
dc.contributor.authorHo J.S.
dc.contributor.authorYang I.H.
dc.date.accessioned2020-03-19T09:02:10Z
dc.date.available2020-03-19T09:02:10Z
dc.date.issued2017
dc.identifier.citationLee H.U., Blasiak A., Agrawal D.R., Loong D.T.B., Thakor N.V., All A.H., Ho J.S., Yang I.H. (2017). Subcellular electrical stimulation of neurons enhances the myelination of axons by oligodendrocytes. PLoS ONE 12 (7) : e0179642. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0179642
dc.identifier.issn1932-6203
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/165789
dc.description.abstractMyelin formation has been identified as a modulator of neural plasticity. New tools are required to investigate the mechanisms by which environmental inputs and neural activity regulate myelination patterns. In this study, we demonstrate a microfluidic compartmentalized culture system with integrated electrical stimulation capabilities that can induce neural activity by whole cell and focal stimulation. A set of electric field simulations was performed to confirm spatial restriction of the electrical input in the compartmentalized culture system. We further demonstrate that electrode localization is a key consideration for generating uniform the stimulation of neuron and oligodendrocytes within the compartments. Using three configurations of the electrodes we tested the effects of subcellular activation of neural activity on distal axon myelination with oligodendrocytes. We further investigated if oligodendrocytes have to be exposed to the electrical field to induce axon myelination. An isolated stimulation of cell bodies and proximal axons had the same effect as an isolated stimulation of distal axons co-cultured with oligodendrocytes, and the two modes had a non-different result than whole cell stimulation. Our platform enabled the demonstration that electrical stimulation enhances oligodendrocyte maturation and myelin formation independent of the input localization and oligodendrocyte exposure to the electrical field. © 2017 Lee et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.publisherPublic Library of Science
dc.sourceUnpaywall 20200320
dc.subjectanimal cell
dc.subjectanimal tissue
dc.subjectArticle
dc.subjectaxon
dc.subjectcell body
dc.subjectcell differentiation
dc.subjectcell isolation
dc.subjectcontrolled study
dc.subjectelectric field
dc.subjectelectrostimulation
dc.subjectembryo
dc.subjectlimit of quantitation
dc.subjectmicrofluidics
dc.subjectmouse
dc.subjectmyelination
dc.subjectnerve cell
dc.subjectnonhuman
dc.subjectoligodendroglia
dc.subjectrat
dc.subjectsimulation
dc.subjectanimal
dc.subjectaxon
dc.subjectcell fractionation
dc.subjectnerve cell
dc.subjectoligodendroglia
dc.subjectphysiology
dc.subjectAnimals
dc.subjectAxons
dc.subjectElectric Stimulation
dc.subjectMicrofluidics
dc.subjectNeurons
dc.subjectOligodendroglia
dc.subjectSubcellular Fractions
dc.typeArticle
dc.contributor.departmentDEPT OF ELECTRICAL & COMPUTER ENGG
dc.contributor.departmentDEPT OF MEDICINE
dc.contributor.departmentLIFE SCIENCES INSTITUTE
dc.description.doi10.1371/journal.pone.0179642
dc.description.sourcetitlePLoS ONE
dc.description.volume12
dc.description.issue7
dc.description.pagee0179642
dc.published.statePublished
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