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Title: An air-supported liquid crystal system for real-time and label-free characterization of phospholipases and their inhibitors
Authors: Hartono, D. 
Bi, X. 
Yang, K.-L. 
Yung, L.-Y.L. 
Issue Date: 9-Oct-2008
Citation: Hartono, D., Bi, X., Yang, K.-L., Yung, L.-Y.L. (2008-10-09). An air-supported liquid crystal system for real-time and label-free characterization of phospholipases and their inhibitors. Advanced Functional Materials 18 (19) : 2938-2945. ScholarBank@NUS Repository.
Abstract: A new air-supported liquid crystal (LC) system for analyzing interfacial phenomena that occur based on the molecular interaction between LCs and adsorbed molecules of interest at the aqueous/LC interface is reported. Compared with existing LC-based detection systems, the miniature system reported here requires less sample and involves simpler preparation. Using this system, the enzymatic hydrolysis of various phospholipases such as phospholipase A2 (PLA2), phospholipase C (PLC) and phospholipase D (PLD) are characterized. The hydrolysis of phospholipid monolayers self-assembled at aqueous/LC interface induces an orientational response from the LCs. As a result, an optical signal that reflects the spatial and temporal distribution of phospholipids during the enzymatic reaction can be generated in a real-time manner. When well-known phospholipase inhibitors are introduced together with respective phospholipases, no orientational response of LCs is observed. In the case of inhibitors MJ33 and compound 48/80, cross-inhibitions among phospholipases are also observed. This work demonstrates that the air-supported LC system provides a facile label-free assay for characterizing phospholipase activities and for screening enzyme inhibitors. It could potentially be useful for different high throughput and cost-effective enzyme screening assays. © 2008 WILEY-VCH Verlag GmbH & Co. KGaA.
Source Title: Advanced Functional Materials
ISSN: 1616301X
DOI: 10.1002/adfm.200800424
Appears in Collections:Staff Publications

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