Please use this identifier to cite or link to this item:
|Title:||An air-supported liquid crystal system for real-time and label-free characterization of phospholipases and their inhibitors|
|Authors:||Hartono, D. |
|Source:||Hartono, D., Bi, X., Yang, K.-L., Yung, L.-Y.L. (2008-10-09). An air-supported liquid crystal system for real-time and label-free characterization of phospholipases and their inhibitors. Advanced Functional Materials 18 (19) : 2938-2945. ScholarBank@NUS Repository. https://doi.org/10.1002/adfm.200800424|
|Abstract:||A new air-supported liquid crystal (LC) system for analyzing interfacial phenomena that occur based on the molecular interaction between LCs and adsorbed molecules of interest at the aqueous/LC interface is reported. Compared with existing LC-based detection systems, the miniature system reported here requires less sample and involves simpler preparation. Using this system, the enzymatic hydrolysis of various phospholipases such as phospholipase A2 (PLA2), phospholipase C (PLC) and phospholipase D (PLD) are characterized. The hydrolysis of phospholipid monolayers self-assembled at aqueous/LC interface induces an orientational response from the LCs. As a result, an optical signal that reflects the spatial and temporal distribution of phospholipids during the enzymatic reaction can be generated in a real-time manner. When well-known phospholipase inhibitors are introduced together with respective phospholipases, no orientational response of LCs is observed. In the case of inhibitors MJ33 and compound 48/80, cross-inhibitions among phospholipases are also observed. This work demonstrates that the air-supported LC system provides a facile label-free assay for characterizing phospholipase activities and for screening enzyme inhibitors. It could potentially be useful for different high throughput and cost-effective enzyme screening assays. © 2008 WILEY-VCH Verlag GmbH & Co. KGaA.|
|Source Title:||Advanced Functional Materials|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Mar 8, 2018
WEB OF SCIENCETM
checked on Nov 22, 2017
checked on Mar 10, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.