Please use this identifier to cite or link to this item: https://doi.org/10.1002/adfm.200800424
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dc.titleAn air-supported liquid crystal system for real-time and label-free characterization of phospholipases and their inhibitors
dc.contributor.authorHartono, D.
dc.contributor.authorBi, X.
dc.contributor.authorYang, K.-L.
dc.contributor.authorYung, L.-Y.L.
dc.date.accessioned2014-10-09T06:43:18Z
dc.date.available2014-10-09T06:43:18Z
dc.date.issued2008-10-09
dc.identifier.citationHartono, D., Bi, X., Yang, K.-L., Yung, L.-Y.L. (2008-10-09). An air-supported liquid crystal system for real-time and label-free characterization of phospholipases and their inhibitors. Advanced Functional Materials 18 (19) : 2938-2945. ScholarBank@NUS Repository. https://doi.org/10.1002/adfm.200800424
dc.identifier.issn1616301X
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/88527
dc.description.abstractA new air-supported liquid crystal (LC) system for analyzing interfacial phenomena that occur based on the molecular interaction between LCs and adsorbed molecules of interest at the aqueous/LC interface is reported. Compared with existing LC-based detection systems, the miniature system reported here requires less sample and involves simpler preparation. Using this system, the enzymatic hydrolysis of various phospholipases such as phospholipase A2 (PLA2), phospholipase C (PLC) and phospholipase D (PLD) are characterized. The hydrolysis of phospholipid monolayers self-assembled at aqueous/LC interface induces an orientational response from the LCs. As a result, an optical signal that reflects the spatial and temporal distribution of phospholipids during the enzymatic reaction can be generated in a real-time manner. When well-known phospholipase inhibitors are introduced together with respective phospholipases, no orientational response of LCs is observed. In the case of inhibitors MJ33 and compound 48/80, cross-inhibitions among phospholipases are also observed. This work demonstrates that the air-supported LC system provides a facile label-free assay for characterizing phospholipase activities and for screening enzyme inhibitors. It could potentially be useful for different high throughput and cost-effective enzyme screening assays. © 2008 WILEY-VCH Verlag GmbH & Co. KGaA.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1002/adfm.200800424
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentCHEMICAL & BIOMOLECULAR ENGINEERING
dc.description.doi10.1002/adfm.200800424
dc.description.sourcetitleAdvanced Functional Materials
dc.description.volume18
dc.description.issue19
dc.description.page2938-2945
dc.description.codenAFMDC
dc.identifier.isiut000260398300011
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