Please use this identifier to cite or link to this item:
|Title:||Combinatorial peptide microarrays for the rapid determination of kinase specificity||Authors:||Uttamchandani, M.
|Issue Date:||Sep-2003||Citation:||Uttamchandani, M., Chan, E.W.S., Chen, G.Y.J., Yao, S.Q. (2003-09). Combinatorial peptide microarrays for the rapid determination of kinase specificity. Bioorganic and Medicinal Chemistry Letters 13 (18) : 2997-3000. ScholarBank@NUS Repository. https://doi.org/10.1016/S0960-894X(03)00633-4||Abstract:||We report a rapid method for profiling of kinases using a strategy that couples the merits of combinatorics (in rapid diversity generation) with the throughput attainable using microarrays (in parallel screening). Alanine-scanning, deletion and positional-scanning peptide libraries of a kinase substrate were synthesized and site-specifically arrayed onto glass slides. The phosphorylation pattern of target sequences detected using fluorescently-labeled antiphosphoamino acid antibodies revealed the substrate preference of the kinase through its activity profile. © 2003 Elsevier Ltd. All rights reserved.||Source Title:||Bioorganic and Medicinal Chemistry Letters||URI:||http://scholarbank.nus.edu.sg/handle/10635/75774||ISSN:||0960894X||DOI:||10.1016/S0960-894X(03)00633-4|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Dec 1, 2020
WEB OF SCIENCETM
checked on Nov 23, 2020
checked on Nov 30, 2020
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.