Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0960-894X(03)00633-4
Title: Combinatorial peptide microarrays for the rapid determination of kinase specificity
Authors: Uttamchandani, M. 
Chan, E.W.S.
Chen, G.Y.J. 
Yao, S.Q. 
Issue Date: Sep-2003
Citation: Uttamchandani, M., Chan, E.W.S., Chen, G.Y.J., Yao, S.Q. (2003-09). Combinatorial peptide microarrays for the rapid determination of kinase specificity. Bioorganic and Medicinal Chemistry Letters 13 (18) : 2997-3000. ScholarBank@NUS Repository. https://doi.org/10.1016/S0960-894X(03)00633-4
Abstract: We report a rapid method for profiling of kinases using a strategy that couples the merits of combinatorics (in rapid diversity generation) with the throughput attainable using microarrays (in parallel screening). Alanine-scanning, deletion and positional-scanning peptide libraries of a kinase substrate were synthesized and site-specifically arrayed onto glass slides. The phosphorylation pattern of target sequences detected using fluorescently-labeled antiphosphoamino acid antibodies revealed the substrate preference of the kinase through its activity profile. © 2003 Elsevier Ltd. All rights reserved.
Source Title: Bioorganic and Medicinal Chemistry Letters
URI: http://scholarbank.nus.edu.sg/handle/10635/75774
ISSN: 0960894X
DOI: 10.1016/S0960-894X(03)00633-4
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