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|Title:||Combinatorial peptide microarrays for the rapid determination of kinase specificity|
|Authors:||Uttamchandani, M. |
|Citation:||Uttamchandani, M., Chan, E.W.S., Chen, G.Y.J., Yao, S.Q. (2003-09). Combinatorial peptide microarrays for the rapid determination of kinase specificity. Bioorganic and Medicinal Chemistry Letters 13 (18) : 2997-3000. ScholarBank@NUS Repository. https://doi.org/10.1016/S0960-894X(03)00633-4|
|Abstract:||We report a rapid method for profiling of kinases using a strategy that couples the merits of combinatorics (in rapid diversity generation) with the throughput attainable using microarrays (in parallel screening). Alanine-scanning, deletion and positional-scanning peptide libraries of a kinase substrate were synthesized and site-specifically arrayed onto glass slides. The phosphorylation pattern of target sequences detected using fluorescently-labeled antiphosphoamino acid antibodies revealed the substrate preference of the kinase through its activity profile. © 2003 Elsevier Ltd. All rights reserved.|
|Source Title:||Bioorganic and Medicinal Chemistry Letters|
|Appears in Collections:||Staff Publications|
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