Please use this identifier to cite or link to this item:
|Title:||Antibody-based fluorescence detection of kinase activity on a peptide array||Authors:||Lesaicherre, M.-L.
|Issue Date:||19-Aug-2002||Citation:||Lesaicherre, M.-L., Uttamchandani, M., Chen, G.Y.J, Yao, S.Q. (2002-08-19). Antibody-based fluorescence detection of kinase activity on a peptide array. Bioorganic and Medicinal Chemistry Letters 12 (16) : 2085-2088. ScholarBank@NUS Repository. https://doi.org/10.1016/S0960-894X(02)00378-5||Abstract:||Peptide-based microarrays allow for high-throughput identification of protein kinase substrates. However, current methods of detecting kinase activity require the use of radioisotopes. We have developed a novel fluorescence-based approach for quantitative detection of peptide phosphorylation on chip using fluorescently-labeled anti-phosphoserine and anti-phosphotyrosine antibodies. This method is sensitive, specific and extremely fast, presenting obvious advantages and may find wider uses in high-throughput kinase screenings. © 2002 Elsevier Science Ltd. All rights reserved.||Source Title:||Bioorganic and Medicinal Chemistry Letters||URI:||http://scholarbank.nus.edu.sg/handle/10635/75583||ISSN:||0960894X||DOI:||10.1016/S0960-894X(02)00378-5|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.