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Title: Antibody-based fluorescence detection of kinase activity on a peptide array
Authors: Lesaicherre, M.-L. 
Uttamchandani, M. 
Chen, G.Y.J 
Yao, S.Q. 
Issue Date: 19-Aug-2002
Citation: Lesaicherre, M.-L., Uttamchandani, M., Chen, G.Y.J, Yao, S.Q. (2002-08-19). Antibody-based fluorescence detection of kinase activity on a peptide array. Bioorganic and Medicinal Chemistry Letters 12 (16) : 2085-2088. ScholarBank@NUS Repository.
Abstract: Peptide-based microarrays allow for high-throughput identification of protein kinase substrates. However, current methods of detecting kinase activity require the use of radioisotopes. We have developed a novel fluorescence-based approach for quantitative detection of peptide phosphorylation on chip using fluorescently-labeled anti-phosphoserine and anti-phosphotyrosine antibodies. This method is sensitive, specific and extremely fast, presenting obvious advantages and may find wider uses in high-throughput kinase screenings. © 2002 Elsevier Science Ltd. All rights reserved.
Source Title: Bioorganic and Medicinal Chemistry Letters
ISSN: 0960894X
DOI: 10.1016/S0960-894X(02)00378-5
Appears in Collections:Staff Publications

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