Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0960-894X(02)00378-5
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dc.titleAntibody-based fluorescence detection of kinase activity on a peptide array
dc.contributor.authorLesaicherre, M.-L.
dc.contributor.authorUttamchandani, M.
dc.contributor.authorChen, G.Y.J
dc.contributor.authorYao, S.Q.
dc.date.accessioned2014-06-23T05:32:07Z
dc.date.available2014-06-23T05:32:07Z
dc.date.issued2002-08-19
dc.identifier.citationLesaicherre, M.-L., Uttamchandani, M., Chen, G.Y.J, Yao, S.Q. (2002-08-19). Antibody-based fluorescence detection of kinase activity on a peptide array. Bioorganic and Medicinal Chemistry Letters 12 (16) : 2085-2088. ScholarBank@NUS Repository. https://doi.org/10.1016/S0960-894X(02)00378-5
dc.identifier.issn0960894X
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/75583
dc.description.abstractPeptide-based microarrays allow for high-throughput identification of protein kinase substrates. However, current methods of detecting kinase activity require the use of radioisotopes. We have developed a novel fluorescence-based approach for quantitative detection of peptide phosphorylation on chip using fluorescently-labeled anti-phosphoserine and anti-phosphotyrosine antibodies. This method is sensitive, specific and extremely fast, presenting obvious advantages and may find wider uses in high-throughput kinase screenings. © 2002 Elsevier Science Ltd. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/S0960-894X(02)00378-5
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentPAEDIATRICS
dc.contributor.departmentCHEMISTRY
dc.description.doi10.1016/S0960-894X(02)00378-5
dc.description.sourcetitleBioorganic and Medicinal Chemistry Letters
dc.description.volume12
dc.description.issue16
dc.description.page2085-2088
dc.description.codenBMCLE
dc.identifier.isiut000177225500005
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