Please use this identifier to cite or link to this item:
|Title:||Antibody-based fluorescence detection of kinase activity on a peptide array|
|Authors:||Lesaicherre, M.-L. |
|Citation:||Lesaicherre, M.-L., Uttamchandani, M., Chen, G.Y.J, Yao, S.Q. (2002-08-19). Antibody-based fluorescence detection of kinase activity on a peptide array. Bioorganic and Medicinal Chemistry Letters 12 (16) : 2085-2088. ScholarBank@NUS Repository. https://doi.org/10.1016/S0960-894X(02)00378-5|
|Abstract:||Peptide-based microarrays allow for high-throughput identification of protein kinase substrates. However, current methods of detecting kinase activity require the use of radioisotopes. We have developed a novel fluorescence-based approach for quantitative detection of peptide phosphorylation on chip using fluorescently-labeled anti-phosphoserine and anti-phosphotyrosine antibodies. This method is sensitive, specific and extremely fast, presenting obvious advantages and may find wider uses in high-throughput kinase screenings. © 2002 Elsevier Science Ltd. All rights reserved.|
|Source Title:||Bioorganic and Medicinal Chemistry Letters|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Sep 21, 2018
WEB OF SCIENCETM
checked on Sep 11, 2018
checked on Aug 17, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.