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|Title:||Antibody-based fluorescence detection of kinase activity on a peptide array|
|Authors:||Lesaicherre, M.-L. |
|Citation:||Lesaicherre, M.-L., Uttamchandani, M., Chen, G.Y.J, Yao, S.Q. (2002-08-19). Antibody-based fluorescence detection of kinase activity on a peptide array. Bioorganic and Medicinal Chemistry Letters 12 (16) : 2085-2088. ScholarBank@NUS Repository. https://doi.org/10.1016/S0960-894X(02)00378-5|
|Abstract:||Peptide-based microarrays allow for high-throughput identification of protein kinase substrates. However, current methods of detecting kinase activity require the use of radioisotopes. We have developed a novel fluorescence-based approach for quantitative detection of peptide phosphorylation on chip using fluorescently-labeled anti-phosphoserine and anti-phosphotyrosine antibodies. This method is sensitive, specific and extremely fast, presenting obvious advantages and may find wider uses in high-throughput kinase screenings. © 2002 Elsevier Science Ltd. All rights reserved.|
|Source Title:||Bioorganic and Medicinal Chemistry Letters|
|Appears in Collections:||Staff Publications|
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