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https://doi.org/10.1016/j.bbrc.2009.12.070
Title: | Structural characterization reveals that viperin is a radical S-adenosyl-l-methionine (SAM) enzyme | Authors: | Shaveta, G. Song, J. Shi, J. Chow, V.T.K. |
Keywords: | Circular dichroism spectroscopy Interferon Iron-sulfur cluster NMR spectroscopy Radical S-adenosyl-l-methionine (SAM) enzyme Viperin |
Issue Date: | 2010 | Citation: | Shaveta, G., Song, J., Shi, J., Chow, V.T.K. (2010). Structural characterization reveals that viperin is a radical S-adenosyl-l-methionine (SAM) enzyme. Biochemical and Biophysical Research Communications 391 (3) : 1390-1395. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bbrc.2009.12.070 | Abstract: | Viperin is an interferon-inducible protein inhibiting many DNA and RNA viruses. It contains an N-terminal transmembrane helix, a highly conserved C-terminus and a middle region carrying a CX3CX2C motif, characteristic of radical S-adenosyl-l-methionine (SAM) enzymes. So far no structural characterization has been reported and reconstitution of the [4Fe-4S] cluster in viperin all failed. Here, by dissecting the 361-residue human viperin into 12 fragments, followed by extensive CD and NMR characterization, Viperin (45-361) was identified to be soluble and structured in buffers. Most importantly, we have successfully reconstituted the [4Fe-4S] cluster in Viperin (45-361), thus providing the first experimental evidence confirming that viperin is indeed a radical SAM enzyme. Furthermore, the C-terminus Viperin (214-361) which is insoluble in buffers but again can be solubilized in salt-free water appears to be only partially folded. Our results thus imply that the radical SAM enzyme activity may play a key role in the broad antiviral actions of viperin. © 2009 Elsevier Inc. All rights reserved. | Source Title: | Biochemical and Biophysical Research Communications | URI: | http://scholarbank.nus.edu.sg/handle/10635/28929 | ISSN: | 0006291X 10902104 |
DOI: | 10.1016/j.bbrc.2009.12.070 |
Appears in Collections: | Staff Publications |
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