Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bbrc.2009.12.070
Title: Structural characterization reveals that viperin is a radical S-adenosyl-l-methionine (SAM) enzyme
Authors: Shaveta, G.
Song, J. 
Shi, J. 
Chow, V.T.K. 
Keywords: Circular dichroism spectroscopy
Interferon
Iron-sulfur cluster
NMR spectroscopy
Radical S-adenosyl-l-methionine (SAM) enzyme
Viperin
Issue Date: 2010
Citation: Shaveta, G., Song, J., Shi, J., Chow, V.T.K. (2010). Structural characterization reveals that viperin is a radical S-adenosyl-l-methionine (SAM) enzyme. Biochemical and Biophysical Research Communications 391 (3) : 1390-1395. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bbrc.2009.12.070
Abstract: Viperin is an interferon-inducible protein inhibiting many DNA and RNA viruses. It contains an N-terminal transmembrane helix, a highly conserved C-terminus and a middle region carrying a CX3CX2C motif, characteristic of radical S-adenosyl-l-methionine (SAM) enzymes. So far no structural characterization has been reported and reconstitution of the [4Fe-4S] cluster in viperin all failed. Here, by dissecting the 361-residue human viperin into 12 fragments, followed by extensive CD and NMR characterization, Viperin (45-361) was identified to be soluble and structured in buffers. Most importantly, we have successfully reconstituted the [4Fe-4S] cluster in Viperin (45-361), thus providing the first experimental evidence confirming that viperin is indeed a radical SAM enzyme. Furthermore, the C-terminus Viperin (214-361) which is insoluble in buffers but again can be solubilized in salt-free water appears to be only partially folded. Our results thus imply that the radical SAM enzyme activity may play a key role in the broad antiviral actions of viperin. © 2009 Elsevier Inc. All rights reserved.
Source Title: Biochemical and Biophysical Research Communications
URI: http://scholarbank.nus.edu.sg/handle/10635/28929
ISSN: 0006291X
10902104
DOI: 10.1016/j.bbrc.2009.12.070
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