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Title: Specific interaction of capsid protein and importin-α/β influences West Nile virus production
Authors: Bhuvanakantham, R. 
Chong, M.-K. 
Ng, M.-L. 
Keywords: Binding strength
Virus production
Issue Date: 2009
Citation: Bhuvanakantham, R., Chong, M.-K., Ng, M.-L. (2009). Specific interaction of capsid protein and importin-α/β influences West Nile virus production. Biochemical and Biophysical Research Communications 389 (1) : 63-69. ScholarBank@NUS Repository.
Abstract: West Nile virus (WNV) capsid (C) protein has been shown to enter the nucleus of infected cells. However, the mechanism by which C protein enters the nucleus is unknown. In this study, we have unveiled for the first time that nuclear transport of WNV and Dengue virus C protein is mediated by their direct association with importin-α. This interplay is mediated by the consensus sequences of bipartite nuclear localization signal located between amino acid residues 85-101 together with amino acid residues 42 and 43 of C protein. Elucidation of biological significance of importin-α/C protein interaction demonstrated that the binding efficiency of this association influenced the nuclear entry of C protein and virus production. Collectively, this study illustrated the molecular mechanism by which the C protein of arthropod-borne flavivirus enters the nucleus and showed the importance of importin-α/C protein interaction in the context of flavivirus life-cycle. © 2009 Elsevier Inc. All rights reserved.
Source Title: Biochemical and Biophysical Research Communications
ISSN: 0006291X
DOI: 10.1016/j.bbrc.2009.08.108
Appears in Collections:Staff Publications

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