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Title: Employment of a high throughput functional assay to define the critical factors that influence vaccine induced cross-variant neutralizing antibodies for SARS-CoV-2
Authors: Gu, Yue 
Shunmuganathan, Bhuvaneshwari 
Qian, Xinlei 
Gupta, Rashi 
Tan, Rebecca SW
Kozma, Mary 
Purushotorman, Kiren 
Murali, Tanusya M 
Tan, Nikki YJ 
Preiser, Peter R
Lescar, Julien
Nasir, Haziq
Somani, Jyoti
Tambyah, Paul A
Fong, Siew-Wai
Amrun, Siti Naqiah
Goh, Yun-Shan
Tay, Matthew Zi-Rui
Rouers, Angeline
Chang, Zi Wei
Yeo, Nicholas Kim-Wah
Chan, Yi-Hao
Hor, Pei Xian
Loh, Chiew Yee
Yang, Yuling
Ruesta, Anthony Torres
Neo, Vanessa
Chen, Wendy Yehui
Goh, Estelle Yi-Wei
Ong, Alice Soh-Meoy
Chua, Adeline Chiew Yen
Nguee, Samantha
Tang, Yong Jie
Tang, Weiyi
Wong, Joel Xu En
Smith, Kenneth GC
Renia, Laurent
Ng, Lisa FP
Lye, David C
Young, Barnaby E
Macary, Paul A 
Keywords: Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
Issue Date: 9-Dec-2023
Citation: Gu, Yue, Shunmuganathan, Bhuvaneshwari, Qian, Xinlei, Gupta, Rashi, Tan, Rebecca SW, Kozma, Mary, Purushotorman, Kiren, Murali, Tanusya M, Tan, Nikki YJ, Preiser, Peter R, Lescar, Julien, Nasir, Haziq, Somani, Jyoti, Tambyah, Paul A, Fong, Siew-Wai, Amrun, Siti Naqiah, Goh, Yun-Shan, Tay, Matthew Zi-Rui, Rouers, Angeline, Chang, Zi Wei, Yeo, Nicholas Kim-Wah, Chan, Yi-Hao, Hor, Pei Xian, Loh, Chiew Yee, Yang, Yuling, Ruesta, Anthony Torres, Neo, Vanessa, Chen, Wendy Yehui, Goh, Estelle Yi-Wei, Ong, Alice Soh-Meoy, Chua, Adeline Chiew Yen, Nguee, Samantha, Tang, Yong Jie, Tang, Weiyi, Wong, Joel Xu En, Smith, Kenneth GC, Renia, Laurent, Ng, Lisa FP, Lye, David C, Young, Barnaby E, Macary, Paul A (2023-12-09). Employment of a high throughput functional assay to define the critical factors that influence vaccine induced cross-variant neutralizing antibodies for SARS-CoV-2. SCIENTIFIC REPORTS 13 (1). ScholarBank@NUS Repository.
Abstract: The scale and duration of neutralizing antibody responses targeting SARS-CoV-2 viral variants represents a critically important serological parameter that predicts protective immunity for COVID-19. In this study, we describe the development and employment of a new functional assay that measures neutralizing antibodies for SARS-CoV-2 and present longitudinal data illustrating the impact of age, sex and comorbidities on the kinetics and strength of vaccine-induced antibody responses for key variants in an Asian volunteer cohort. We also present an accurate quantitation of serological responses for SARS-CoV-2 that exploits a unique set of in-house, recombinant human monoclonal antibodies targeting the viral Spike and nucleocapsid proteins and demonstrate a reduction in neutralizing antibody titres across all groups 6 months post-vaccination. We also observe a marked reduction in the serological binding activity and neutralizing responses targeting recently newly emerged Omicron variants including XBB 1.5 and highlight a significant increase in cross-protective neutralizing antibody responses following a third dose (boost) of vaccine. These data illustrate how key virological factors such as immune escape mutations combined with host demographic factors such as age and sex of the vaccinated individual influence the strength and duration of cross-protective serological immunity for COVID-19.
ISSN: 2045-2322
DOI: 10.1038/s41598-023-49231-w
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