Please use this identifier to cite or link to this item:
https://doi.org/10.1038/s41598-023-49231-w
DC Field | Value | |
---|---|---|
dc.title | Employment of a high throughput functional assay to define the critical factors that influence vaccine induced cross-variant neutralizing antibodies for SARS-CoV-2 | |
dc.contributor.author | Gu, Yue | |
dc.contributor.author | Shunmuganathan, Bhuvaneshwari | |
dc.contributor.author | Qian, Xinlei | |
dc.contributor.author | Gupta, Rashi | |
dc.contributor.author | Tan, Rebecca SW | |
dc.contributor.author | Kozma, Mary | |
dc.contributor.author | Purushotorman, Kiren | |
dc.contributor.author | Murali, Tanusya M | |
dc.contributor.author | Tan, Nikki YJ | |
dc.contributor.author | Preiser, Peter R | |
dc.contributor.author | Lescar, Julien | |
dc.contributor.author | Nasir, Haziq | |
dc.contributor.author | Somani, Jyoti | |
dc.contributor.author | Tambyah, Paul A | |
dc.contributor.author | Fong, Siew-Wai | |
dc.contributor.author | Amrun, Siti Naqiah | |
dc.contributor.author | Goh, Yun-Shan | |
dc.contributor.author | Tay, Matthew Zi-Rui | |
dc.contributor.author | Rouers, Angeline | |
dc.contributor.author | Chang, Zi Wei | |
dc.contributor.author | Yeo, Nicholas Kim-Wah | |
dc.contributor.author | Chan, Yi-Hao | |
dc.contributor.author | Hor, Pei Xian | |
dc.contributor.author | Loh, Chiew Yee | |
dc.contributor.author | Yang, Yuling | |
dc.contributor.author | Ruesta, Anthony Torres | |
dc.contributor.author | Neo, Vanessa | |
dc.contributor.author | Chen, Wendy Yehui | |
dc.contributor.author | Goh, Estelle Yi-Wei | |
dc.contributor.author | Ong, Alice Soh-Meoy | |
dc.contributor.author | Chua, Adeline Chiew Yen | |
dc.contributor.author | Nguee, Samantha | |
dc.contributor.author | Tang, Yong Jie | |
dc.contributor.author | Tang, Weiyi | |
dc.contributor.author | Wong, Joel Xu En | |
dc.contributor.author | Smith, Kenneth GC | |
dc.contributor.author | Renia, Laurent | |
dc.contributor.author | Ng, Lisa FP | |
dc.contributor.author | Lye, David C | |
dc.contributor.author | Young, Barnaby E | |
dc.contributor.author | Macary, Paul A | |
dc.date.accessioned | 2024-02-01T05:53:21Z | |
dc.date.available | 2024-02-01T05:53:21Z | |
dc.date.issued | 2023-12-09 | |
dc.identifier.citation | Gu, Yue, Shunmuganathan, Bhuvaneshwari, Qian, Xinlei, Gupta, Rashi, Tan, Rebecca SW, Kozma, Mary, Purushotorman, Kiren, Murali, Tanusya M, Tan, Nikki YJ, Preiser, Peter R, Lescar, Julien, Nasir, Haziq, Somani, Jyoti, Tambyah, Paul A, Fong, Siew-Wai, Amrun, Siti Naqiah, Goh, Yun-Shan, Tay, Matthew Zi-Rui, Rouers, Angeline, Chang, Zi Wei, Yeo, Nicholas Kim-Wah, Chan, Yi-Hao, Hor, Pei Xian, Loh, Chiew Yee, Yang, Yuling, Ruesta, Anthony Torres, Neo, Vanessa, Chen, Wendy Yehui, Goh, Estelle Yi-Wei, Ong, Alice Soh-Meoy, Chua, Adeline Chiew Yen, Nguee, Samantha, Tang, Yong Jie, Tang, Weiyi, Wong, Joel Xu En, Smith, Kenneth GC, Renia, Laurent, Ng, Lisa FP, Lye, David C, Young, Barnaby E, Macary, Paul A (2023-12-09). Employment of a high throughput functional assay to define the critical factors that influence vaccine induced cross-variant neutralizing antibodies for SARS-CoV-2. SCIENTIFIC REPORTS 13 (1). ScholarBank@NUS Repository. https://doi.org/10.1038/s41598-023-49231-w | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/246944 | |
dc.description.abstract | The scale and duration of neutralizing antibody responses targeting SARS-CoV-2 viral variants represents a critically important serological parameter that predicts protective immunity for COVID-19. In this study, we describe the development and employment of a new functional assay that measures neutralizing antibodies for SARS-CoV-2 and present longitudinal data illustrating the impact of age, sex and comorbidities on the kinetics and strength of vaccine-induced antibody responses for key variants in an Asian volunteer cohort. We also present an accurate quantitation of serological responses for SARS-CoV-2 that exploits a unique set of in-house, recombinant human monoclonal antibodies targeting the viral Spike and nucleocapsid proteins and demonstrate a reduction in neutralizing antibody titres across all groups 6 months post-vaccination. We also observe a marked reduction in the serological binding activity and neutralizing responses targeting recently newly emerged Omicron variants including XBB 1.5 and highlight a significant increase in cross-protective neutralizing antibody responses following a third dose (boost) of vaccine. These data illustrate how key virological factors such as immune escape mutations combined with host demographic factors such as age and sex of the vaccinated individual influence the strength and duration of cross-protective serological immunity for COVID-19. | |
dc.language.iso | en | |
dc.publisher | NATURE PORTFOLIO | |
dc.source | Elements | |
dc.subject | Science & Technology | |
dc.subject | Multidisciplinary Sciences | |
dc.subject | Science & Technology - Other Topics | |
dc.subject | MEMORY | |
dc.type | Article | |
dc.date.updated | 2024-02-01T05:45:14Z | |
dc.contributor.department | MICROBIOLOGY AND IMMUNOLOGY | |
dc.contributor.department | LIFE SCIENCES INSTITUTE | |
dc.description.doi | 10.1038/s41598-023-49231-w | |
dc.description.sourcetitle | SCIENTIFIC REPORTS | |
dc.description.volume | 13 | |
dc.description.issue | 1 | |
dc.published.state | Published | |
Appears in Collections: | Elements Staff Publications |
Show simple item record
Files in This Item:
File | Description | Size | Format | Access Settings | Version | |
---|---|---|---|---|---|---|
41598_2023_Article_49231.pdf | 5.37 MB | Adobe PDF | OPEN | Published | View/Download |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.