Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.str.2018.12.006
Title: Infectivity of Dengue Virus Serotypes 1 and 2 Is Correlated with E-Protein Intrinsic Dynamics but Not to Envelope Conformations
Authors: Sharma, Kamal Kant 
Lim, Xin-Xiang 
Tantirimudalige, Sarala Neomi 
Gupta, Anjali 
Marzinek, Jan K 
Holdbrook, Daniel
Lim, Xin Ying Elisa 
Bond, Peter J 
Anand, Ganesh S 
Wohland, Thorsten 
Keywords: Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
Biophysics
Cell Biology
HYDROGEN-EXCHANGE
MASS-SPECTROMETRY
FORCE-FIELD
TEMPERATURE
ANTIBODIES
NEUTRALIZATION
GLYCOPROTEIN
ORGANIZATION
TRANSITIONS
SIMULATIONS
Issue Date: 2-Apr-2019
Publisher: CELL PRESS
Citation: Sharma, Kamal Kant, Lim, Xin-Xiang, Tantirimudalige, Sarala Neomi, Gupta, Anjali, Marzinek, Jan K, Holdbrook, Daniel, Lim, Xin Ying Elisa, Bond, Peter J, Anand, Ganesh S, Wohland, Thorsten (2019-04-02). Infectivity of Dengue Virus Serotypes 1 and 2 Is Correlated with E-Protein Intrinsic Dynamics but Not to Envelope Conformations. STRUCTURE 27 (4) : 618-+. ScholarBank@NUS Repository. https://doi.org/10.1016/j.str.2018.12.006
Abstract: Dengue is a mosquito-borne virus with dire health and economic impacts. Dengue is responsible for an estimated 390 million infections per year, with dengue 2 (DENV2) being the most virulent strain among the four serotypes. Interestingly, it is also in strains of this serotype that temperature-dependent large-scale morphological changes, termed “breathing,” have been observed. Although the structure of these morphologies has been solved to 3.5-Å resolution, the dynamics of the viral envelope are unknown. Here, we combine fluorescence and mass spectrometry with molecular dynamics simulations to provide insights into DENV2 (NGC strain) structural dynamics in comparison with DENV1 (PVP 159). We observe hitherto unseen conformational changes and structural dynamics of the DENV2 envelope that are influenced by both temperature and divalent cations. Our results show that for DENV2 and DENV1 the intrinsic dynamics, but not the specific morphologies, are correlated with viral infectivity.
Source Title: STRUCTURE
URI: https://scholarbank.nus.edu.sg/handle/10635/242843
ISSN: 0969-2126
1878-4186
DOI: 10.1016/j.str.2018.12.006
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