Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.str.2018.12.006
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dc.titleInfectivity of Dengue Virus Serotypes 1 and 2 Is Correlated with E-Protein Intrinsic Dynamics but Not to Envelope Conformations
dc.contributor.authorSharma, Kamal Kant
dc.contributor.authorLim, Xin-Xiang
dc.contributor.authorTantirimudalige, Sarala Neomi
dc.contributor.authorGupta, Anjali
dc.contributor.authorMarzinek, Jan K
dc.contributor.authorHoldbrook, Daniel
dc.contributor.authorLim, Xin Ying Elisa
dc.contributor.authorBond, Peter J
dc.contributor.authorAnand, Ganesh S
dc.contributor.authorWohland, Thorsten
dc.date.accessioned2023-07-06T09:32:17Z
dc.date.available2023-07-06T09:32:17Z
dc.date.issued2019-04-02
dc.identifier.citationSharma, Kamal Kant, Lim, Xin-Xiang, Tantirimudalige, Sarala Neomi, Gupta, Anjali, Marzinek, Jan K, Holdbrook, Daniel, Lim, Xin Ying Elisa, Bond, Peter J, Anand, Ganesh S, Wohland, Thorsten (2019-04-02). Infectivity of Dengue Virus Serotypes 1 and 2 Is Correlated with E-Protein Intrinsic Dynamics but Not to Envelope Conformations. STRUCTURE 27 (4) : 618-+. ScholarBank@NUS Repository. https://doi.org/10.1016/j.str.2018.12.006
dc.identifier.issn0969-2126
dc.identifier.issn1878-4186
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/242843
dc.description.abstractDengue is a mosquito-borne virus with dire health and economic impacts. Dengue is responsible for an estimated 390 million infections per year, with dengue 2 (DENV2) being the most virulent strain among the four serotypes. Interestingly, it is also in strains of this serotype that temperature-dependent large-scale morphological changes, termed “breathing,” have been observed. Although the structure of these morphologies has been solved to 3.5-Å resolution, the dynamics of the viral envelope are unknown. Here, we combine fluorescence and mass spectrometry with molecular dynamics simulations to provide insights into DENV2 (NGC strain) structural dynamics in comparison with DENV1 (PVP 159). We observe hitherto unseen conformational changes and structural dynamics of the DENV2 envelope that are influenced by both temperature and divalent cations. Our results show that for DENV2 and DENV1 the intrinsic dynamics, but not the specific morphologies, are correlated with viral infectivity.
dc.language.isoen
dc.publisherCELL PRESS
dc.sourceElements
dc.subjectScience & Technology
dc.subjectLife Sciences & Biomedicine
dc.subjectBiochemistry & Molecular Biology
dc.subjectBiophysics
dc.subjectCell Biology
dc.subjectHYDROGEN-EXCHANGE
dc.subjectMASS-SPECTROMETRY
dc.subjectFORCE-FIELD
dc.subjectTEMPERATURE
dc.subjectANTIBODIES
dc.subjectNEUTRALIZATION
dc.subjectGLYCOPROTEIN
dc.subjectORGANIZATION
dc.subjectTRANSITIONS
dc.subjectSIMULATIONS
dc.typeArticle
dc.date.updated2023-07-06T08:07:30Z
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.contributor.departmentDUKE-NUS MEDICAL SCHOOL
dc.description.doi10.1016/j.str.2018.12.006
dc.description.sourcetitleSTRUCTURE
dc.description.volume27
dc.description.issue4
dc.description.page618-+
dc.published.statePublished
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