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Title: A rapid simple point-of-care assay for the detection of SARS-CoV-2 neutralizing antibodies
Authors: Kongsuphol, Patthara
Jia, Huan
Cheng, Hoi Lok
Gu, Yue 
Shunmuganathan, Bhuvaneshwari 
Chen, Ming Wei
Lim, Sing Mei
Ng, Say Yong
Tambyah, Paul Ananth
Nasir, Haziq 
Gao, Xiaohong
Tay, Dousabel
Kim, Seunghyeon
Gupta, Rashi 
Qian, Xinlei 
Kozma, Mary M 
Purushotorman, Kiren 
McBee, Megan E
MacAry, Paul A 
Sikes, Hadley D
Preiser, Peter R
Keywords: Science & Technology
Life Sciences & Biomedicine
Medicine, Research & Experimental
Research & Experimental Medicine
Issue Date: 11-Nov-2021
Citation: Kongsuphol, Patthara, Jia, Huan, Cheng, Hoi Lok, Gu, Yue, Shunmuganathan, Bhuvaneshwari, Chen, Ming Wei, Lim, Sing Mei, Ng, Say Yong, Tambyah, Paul Ananth, Nasir, Haziq, Gao, Xiaohong, Tay, Dousabel, Kim, Seunghyeon, Gupta, Rashi, Qian, Xinlei, Kozma, Mary M, Purushotorman, Kiren, McBee, Megan E, MacAry, Paul A, Sikes, Hadley D, Preiser, Peter R (2021-11-11). A rapid simple point-of-care assay for the detection of SARS-CoV-2 neutralizing antibodies. COMMUNICATIONS MEDICINE 1 (1). ScholarBank@NUS Repository.
Abstract: BACKGROUND: Neutralizing antibodies (NAbs) prevent pathogens from infecting host cells. Detection of SARS-CoV-2 NAbs is critical to evaluate herd immunity and monitor vaccine efficacy against SARS-CoV-2, the virus that causes COVID-19. All currently available NAb tests are lab-based and time-intensive. METHOD: We develop a 10 min cellulose pull-down test to detect NAbs against SARS-CoV-2 from human plasma. The test evaluates the ability of antibodies to disrupt ACE2 receptor-RBD complex formation. The simple, portable, and rapid testing process relies on two key technologies: (i) the vertical-flow paper-based assay format and (ii) the rapid interaction of cellulose binding domain to cellulose paper. RESULTS: Here we show the construction of a cellulose-based vertical-flow test. The developed test gives above 80% sensitivity and specificity and up to 93% accuracy as compared to two current lab-based methods using COVID-19 convalescent plasma. CONCLUSIONS: A rapid 10 min cellulose based test has been developed for detection of NAb against SARS-CoV-2. The test demonstrates comparable performance to the lab-based tests and can be used at Point-of-Care. Importantly, the approach used for this test can be easily extended to test RBD variants or to evaluate NAbs against other pathogens.
ISSN: 2730-664X
DOI: 10.1038/s43856-021-00045-9
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