Please use this identifier to cite or link to this item: https://doi.org/10.1038/s43856-021-00045-9
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dc.titleA rapid simple point-of-care assay for the detection of SARS-CoV-2 neutralizing antibodies
dc.contributor.authorKongsuphol, Patthara
dc.contributor.authorJia, Huan
dc.contributor.authorCheng, Hoi Lok
dc.contributor.authorGu, Yue
dc.contributor.authorShunmuganathan, Bhuvaneshwari
dc.contributor.authorChen, Ming Wei
dc.contributor.authorLim, Sing Mei
dc.contributor.authorNg, Say Yong
dc.contributor.authorTambyah, Paul Ananth
dc.contributor.authorNasir, Haziq
dc.contributor.authorGao, Xiaohong
dc.contributor.authorTay, Dousabel
dc.contributor.authorKim, Seunghyeon
dc.contributor.authorGupta, Rashi
dc.contributor.authorQian, Xinlei
dc.contributor.authorKozma, Mary M
dc.contributor.authorPurushotorman, Kiren
dc.contributor.authorMcBee, Megan E
dc.contributor.authorMacAry, Paul A
dc.contributor.authorSikes, Hadley D
dc.contributor.authorPreiser, Peter R
dc.date.accessioned2023-05-18T08:15:42Z
dc.date.available2023-05-18T08:15:42Z
dc.date.issued2021-11-11
dc.identifier.citationKongsuphol, Patthara, Jia, Huan, Cheng, Hoi Lok, Gu, Yue, Shunmuganathan, Bhuvaneshwari, Chen, Ming Wei, Lim, Sing Mei, Ng, Say Yong, Tambyah, Paul Ananth, Nasir, Haziq, Gao, Xiaohong, Tay, Dousabel, Kim, Seunghyeon, Gupta, Rashi, Qian, Xinlei, Kozma, Mary M, Purushotorman, Kiren, McBee, Megan E, MacAry, Paul A, Sikes, Hadley D, Preiser, Peter R (2021-11-11). A rapid simple point-of-care assay for the detection of SARS-CoV-2 neutralizing antibodies. COMMUNICATIONS MEDICINE 1 (1). ScholarBank@NUS Repository. https://doi.org/10.1038/s43856-021-00045-9
dc.identifier.issn2730-664X
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/239497
dc.description.abstractBACKGROUND: Neutralizing antibodies (NAbs) prevent pathogens from infecting host cells. Detection of SARS-CoV-2 NAbs is critical to evaluate herd immunity and monitor vaccine efficacy against SARS-CoV-2, the virus that causes COVID-19. All currently available NAb tests are lab-based and time-intensive. METHOD: We develop a 10 min cellulose pull-down test to detect NAbs against SARS-CoV-2 from human plasma. The test evaluates the ability of antibodies to disrupt ACE2 receptor-RBD complex formation. The simple, portable, and rapid testing process relies on two key technologies: (i) the vertical-flow paper-based assay format and (ii) the rapid interaction of cellulose binding domain to cellulose paper. RESULTS: Here we show the construction of a cellulose-based vertical-flow test. The developed test gives above 80% sensitivity and specificity and up to 93% accuracy as compared to two current lab-based methods using COVID-19 convalescent plasma. CONCLUSIONS: A rapid 10 min cellulose based test has been developed for detection of NAb against SARS-CoV-2. The test demonstrates comparable performance to the lab-based tests and can be used at Point-of-Care. Importantly, the approach used for this test can be easily extended to test RBD variants or to evaluate NAbs against other pathogens.
dc.language.isoen
dc.publisherSPRINGERNATURE
dc.sourceElements
dc.subjectScience & Technology
dc.subjectLife Sciences & Biomedicine
dc.subjectMedicine, Research & Experimental
dc.subjectResearch & Experimental Medicine
dc.subjectSARS CORONAVIRUS
dc.subjectPROTEIN
dc.subjectSPIKE
dc.subjectEFFICIENT
dc.subjectIMMUNITY
dc.subjectBINDING
dc.subjectLEVEL
dc.typeArticle
dc.date.updated2023-05-18T03:51:06Z
dc.contributor.departmentMICROBIOLOGY AND IMMUNOLOGY
dc.contributor.departmentLIFE SCIENCES INSTITUTE
dc.description.doi10.1038/s43856-021-00045-9
dc.description.sourcetitleCOMMUNICATIONS MEDICINE
dc.description.volume1
dc.description.issue1
dc.published.statePublished
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