Please use this identifier to cite or link to this item:
https://doi.org/10.21769/BioProtoc.3012
| Title: | Identifying Protein Interactions with Histone Peptides Using Bio-layer Interferometry. | Authors: | Ren, Bingbing Sayed, Ahmed Mahmoud Mohammed Tan, Hwei Ling Mok, Yu Keung Chen, Ee Sin |
Keywords: | Bio-layer interferometry Biosensor Histone post-translational modification Label free Peptide Protein interaction Recombinant protein |
Issue Date: | 20-Sep-2018 | Publisher: | Bio-Protocol, LLC | Citation: | Ren, Bingbing, Sayed, Ahmed Mahmoud Mohammed, Tan, Hwei Ling, Mok, Yu Keung, Chen, Ee Sin (2018-09-20). Identifying Protein Interactions with Histone Peptides Using Bio-layer Interferometry.. Bio Protoc 8 (18) : e3012-. ScholarBank@NUS Repository. https://doi.org/10.21769/BioProtoc.3012 | Abstract: | Histone post-translational modifications (PTMs) regulate numerous cellular processes, including gene transcription, cell division, and DNA damage repair. Most histone PTMs affect the recruitment or exclusion of reader proteins from chromatin. Here, we present a protocol to measure affinity and interaction kinetics between histone peptides and the recombinant protein using Bio-layer interferometry. | Source Title: | Bio Protoc | URI: | https://scholarbank.nus.edu.sg/handle/10635/239186 | ISSN: | 2331-8325 | DOI: | 10.21769/BioProtoc.3012 |
| Appears in Collections: | Elements Staff Publications |
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| File | Description | Size | Format | Access Settings | Version | |
|---|---|---|---|---|---|---|
| 2018 Ren et al Bio-Protocol.pdf | 9.02 MB | Adobe PDF | OPEN | None | View/Download |
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