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Please use this identifier to cite or link to this item: https://doi.org/10.21769/BioProtoc.3012
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dc.titleIdentifying Protein Interactions with Histone Peptides Using Bio-layer Interferometry.
dc.contributor.authorRen, Bingbing
dc.contributor.authorSayed, Ahmed Mahmoud Mohammed
dc.contributor.authorTan, Hwei Ling
dc.contributor.authorMok, Yu Keung
dc.contributor.authorChen, Ee Sin
dc.date.accessioned2023-05-04T04:41:36Z
dc.date.available2023-05-04T04:41:36Z
dc.date.issued2018-09-20
dc.identifier.citationRen, Bingbing, Sayed, Ahmed Mahmoud Mohammed, Tan, Hwei Ling, Mok, Yu Keung, Chen, Ee Sin (2018-09-20). Identifying Protein Interactions with Histone Peptides Using Bio-layer Interferometry.. Bio Protoc 8 (18) : e3012-. ScholarBank@NUS Repository. https://doi.org/10.21769/BioProtoc.3012
dc.identifier.issn2331-8325
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/239186
dc.description.abstractHistone post-translational modifications (PTMs) regulate numerous cellular processes, including gene transcription, cell division, and DNA damage repair. Most histone PTMs affect the recruitment or exclusion of reader proteins from chromatin. Here, we present a protocol to measure affinity and interaction kinetics between histone peptides and the recombinant protein using Bio-layer interferometry.
dc.publisherBio-Protocol, LLC
dc.sourceElements
dc.subjectBio-layer interferometry
dc.subjectBiosensor
dc.subjectHistone post-translational modification
dc.subjectLabel free
dc.subjectPeptide
dc.subjectProtein interaction
dc.subjectRecombinant protein
dc.typeArticle
dc.date.updated2023-05-02T07:55:38Z
dc.contributor.departmentBIOCHEMISTRY
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.21769/BioProtoc.3012
dc.description.sourcetitleBio Protoc
dc.description.volume8
dc.description.issue18
dc.description.pagee3012-
dc.published.statePublished online
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