Please use this identifier to cite or link to this item: https://doi.org/10.18632/aging.101389
Title: Do senescence markers correlate in vitro and in situ within individual human donors?
Authors: Waaijer, Mariette EC
Gunn, David A
van Heemst, Diana
Slagboom, P Eline
Sedivy, John M
Dirks, Roeland W
Tanke, Hans J
Westendorp, Rudi GJ
Maier, Andrea B 
Keywords: Science & Technology
Life Sciences & Biomedicine
Cell Biology
Geriatrics & Gerontology
cellular senescence
correlation
markers
in vitro
in situ
STRESS-INDUCED RESPONSES
DIPLOID CELL STRAINS
REPLICATIVE CAPACITY
DERMAL FIBROBLASTS
BETA-GALACTOSIDASE
BIOLOGICAL AGE
VIVO
IDENTIFICATION
CONTRIBUTES
CULTIVATION
Issue Date: 1-Feb-2018
Publisher: IMPACT JOURNALS LLC
Citation: Waaijer, Mariette EC, Gunn, David A, van Heemst, Diana, Slagboom, P Eline, Sedivy, John M, Dirks, Roeland W, Tanke, Hans J, Westendorp, Rudi GJ, Maier, Andrea B (2018-02-01). Do senescence markers correlate in vitro and in situ within individual human donors?. AGING-US 10 (2) : 278-289. ScholarBank@NUS Repository. https://doi.org/10.18632/aging.101389
Abstract: Little is known on how well senescence markers in vitro and in situ correlate within individual donors. We studied correlations between the same and different in vitro markers. Furthermore, we tested correlations between in vitro markers with in situ p16INK4a positivity. From 100 donors (20-91 years), cultured dermal fibroblasts were assessed for reactive oxygen species (ROS), telomere-associated foci (TAF), p16INK4a and senescence-associated β-gal (SAβ-gal), with/without 0.6 μM rotenone for 3 days (short-term). In fibroblasts from 40 donors, telomere shortening, ROS and SAβ-gal were additionally assessed, with/without 20 nM rotenone for 7 weeks (long-term). In skin from 52 donors, the number of p16INK4a positive dermal cells was assessed in situ. More than half of the correlations of the same senescence markers in vitro between duplicate experiments and between short-term versus long-term experiments were significant. Half of the different senescence marker correlations were significant within the short-term and within the long-term experiments. The different senescence markers in vitro were not significantly correlated intra-individually with in situ p16INK4a positivity. In conclusion, the same and different senescence markers are frequently correlated significantly within and between in vitro experiments, but in vitro senescence markers are not correlated with p16INK4a positivity in situ.
Source Title: AGING-US
URI: https://scholarbank.nus.edu.sg/handle/10635/234940
ISSN: 1945-4589
DOI: 10.18632/aging.101389
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