Please use this identifier to cite or link to this item: https://doi.org/10.1002/btm2.10293
Title: Finger stick blood test to assess postvaccination SARS-CoV-2 neutralizing antibody response against variants
Authors: Lim, Sing Mei
Cheng, Hoi Lok
Jia, Huan
Kongsuphol, Patthara
Shunmuganathan, Bhuvaneshwari 
Chen, Ming Wei
Ng, Say Yong
Gao, Xiaohong
Turaga, Shuvan Prashant
Heussler, Sascha P 
Somani, Jyoti 
Sengupta, Sharmila
Tay, Dousabel MY
McBee, Megan E
Young, Barnaby E
MacAry, Paul A 
Sikes, Hadley D
Preiser, Peter R
Keywords: Science & Technology
Life Sciences & Biomedicine
Technology
Biotechnology & Applied Microbiology
Engineering, Biomedical
Pharmacology & Pharmacy
Engineering
cellulose pulldown assay
COVID19
humoral response against COVID19 variants
neutralizing antibody
point-of-care test
SARS-CoV-2
serology test
VACCINE
Issue Date: 10-Feb-2022
Publisher: WILEY
Citation: Lim, Sing Mei, Cheng, Hoi Lok, Jia, Huan, Kongsuphol, Patthara, Shunmuganathan, Bhuvaneshwari, Chen, Ming Wei, Ng, Say Yong, Gao, Xiaohong, Turaga, Shuvan Prashant, Heussler, Sascha P, Somani, Jyoti, Sengupta, Sharmila, Tay, Dousabel MY, McBee, Megan E, Young, Barnaby E, MacAry, Paul A, Sikes, Hadley D, Preiser, Peter R (2022-02-10). Finger stick blood test to assess postvaccination SARS-CoV-2 neutralizing antibody response against variants. BIOENGINEERING & TRANSLATIONAL MEDICINE 7 (2). ScholarBank@NUS Repository. https://doi.org/10.1002/btm2.10293
Abstract: There is clinical need for a quantifiable point-of-care (PoC) SARS-CoV-2 neutralizing antibody (nAb) test that is adaptable with the pandemic's changing landscape. Here, we present a rapid and semi-quantitative nAb test that uses finger stick or venous blood to assess the nAb response of vaccinated population against wild-type (WT), alpha, beta, gamma, and delta variant RBDs. It captures a clinically relevant range of nAb levels, and effectively differentiates prevaccination, post first dose, and post second dose vaccination samples within 10 min. The data observed against alpha, beta, gamma, and delta variants agrees with published results evaluated in established serology tests. Finally, our test revealed a substantial reduction in nAb level for beta, gamma, and delta variants between early BNT162b2 vaccination group (within 3 months) and later vaccination group (post 3 months). This test is highly suited for PoC settings and provides an insightful nAb response in a postvaccinated population.
Source Title: BIOENGINEERING & TRANSLATIONAL MEDICINE
URI: https://scholarbank.nus.edu.sg/handle/10635/234733
ISSN: 2380-6761
DOI: 10.1002/btm2.10293
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