Please use this identifier to cite or link to this item: https://doi.org/10.1002/btm2.10293
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dc.titleFinger stick blood test to assess postvaccination SARS-CoV-2 neutralizing antibody response against variants
dc.contributor.authorLim, Sing Mei
dc.contributor.authorCheng, Hoi Lok
dc.contributor.authorJia, Huan
dc.contributor.authorKongsuphol, Patthara
dc.contributor.authorShunmuganathan, Bhuvaneshwari
dc.contributor.authorChen, Ming Wei
dc.contributor.authorNg, Say Yong
dc.contributor.authorGao, Xiaohong
dc.contributor.authorTuraga, Shuvan Prashant
dc.contributor.authorHeussler, Sascha P
dc.contributor.authorSomani, Jyoti
dc.contributor.authorSengupta, Sharmila
dc.contributor.authorTay, Dousabel MY
dc.contributor.authorMcBee, Megan E
dc.contributor.authorYoung, Barnaby E
dc.contributor.authorMacAry, Paul A
dc.contributor.authorSikes, Hadley D
dc.contributor.authorPreiser, Peter R
dc.date.accessioned2022-11-21T09:02:59Z
dc.date.available2022-11-21T09:02:59Z
dc.date.issued2022-02-10
dc.identifier.citationLim, Sing Mei, Cheng, Hoi Lok, Jia, Huan, Kongsuphol, Patthara, Shunmuganathan, Bhuvaneshwari, Chen, Ming Wei, Ng, Say Yong, Gao, Xiaohong, Turaga, Shuvan Prashant, Heussler, Sascha P, Somani, Jyoti, Sengupta, Sharmila, Tay, Dousabel MY, McBee, Megan E, Young, Barnaby E, MacAry, Paul A, Sikes, Hadley D, Preiser, Peter R (2022-02-10). Finger stick blood test to assess postvaccination SARS-CoV-2 neutralizing antibody response against variants. BIOENGINEERING & TRANSLATIONAL MEDICINE 7 (2). ScholarBank@NUS Repository. https://doi.org/10.1002/btm2.10293
dc.identifier.issn2380-6761
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/234733
dc.description.abstractThere is clinical need for a quantifiable point-of-care (PoC) SARS-CoV-2 neutralizing antibody (nAb) test that is adaptable with the pandemic's changing landscape. Here, we present a rapid and semi-quantitative nAb test that uses finger stick or venous blood to assess the nAb response of vaccinated population against wild-type (WT), alpha, beta, gamma, and delta variant RBDs. It captures a clinically relevant range of nAb levels, and effectively differentiates prevaccination, post first dose, and post second dose vaccination samples within 10 min. The data observed against alpha, beta, gamma, and delta variants agrees with published results evaluated in established serology tests. Finally, our test revealed a substantial reduction in nAb level for beta, gamma, and delta variants between early BNT162b2 vaccination group (within 3 months) and later vaccination group (post 3 months). This test is highly suited for PoC settings and provides an insightful nAb response in a postvaccinated population.
dc.language.isoen
dc.publisherWILEY
dc.sourceElements
dc.subjectScience & Technology
dc.subjectLife Sciences & Biomedicine
dc.subjectTechnology
dc.subjectBiotechnology & Applied Microbiology
dc.subjectEngineering, Biomedical
dc.subjectPharmacology & Pharmacy
dc.subjectEngineering
dc.subjectcellulose pulldown assay
dc.subjectCOVID19
dc.subjecthumoral response against COVID19 variants
dc.subjectneutralizing antibody
dc.subjectpoint-of-care test
dc.subjectSARS-CoV-2
dc.subjectserology test
dc.subjectVACCINE
dc.typeArticle
dc.date.updated2022-11-19T06:35:37Z
dc.contributor.departmentDEPT OF MICROBIOLOGY & IMMUNOLOGY
dc.contributor.departmentSINGAPORE SYNCHROTRON LIGHT SOURCE
dc.contributor.departmentDEPT OF MEDICINE
dc.description.doi10.1002/btm2.10293
dc.description.sourcetitleBIOENGINEERING & TRANSLATIONAL MEDICINE
dc.description.volume7
dc.description.issue2
dc.published.statePublished
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