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Title: Microbial exposure during early human development primes fetal immune cells
Authors: Mishra, Archita
Lai, Ghee Chuan
Yao, Leong Jing 
Aung, Thet Tun
Shental, Noam
Rotter-Maskowitz, Aviva
Shepherdson, Edwin
Singh, Gurmit Singh Naranjan
Pai, Rhea
Shanti, Adhika
Wong, Regina Men Men
Lee, Andrea
Khyriem, Costerwell
Dutertre, Charles Antoine
Chakarov, Svetoslav
Srinivasan, K. G.
Shadan, Nurhidaya Binte
Zhang, Xiao-Meng
Khalilnezhad, Shabnam
Cottier, Fabien
Tan, Alrina Shin Min
Low, Gillian
Chen, Phyllis
Fan, Yiping
Hor, Pei Xiang
Lee, Avery Khoo May
Choolani, Mahesh 
Vermijlen, David
Sharma, Ankur
Fuks, Garold
Straussman, Ravid
Pavelka, Norman
Malleret, Benoit 
McGovern, Naomi
Albani, Salvatore 
Chan, Jerry Kok Yen 
Ginhoux, Florent 
Keywords: bacteria
fetal Development
fetal immunity
immune memory
immune priming
Issue Date: 1-Jun-2021
Publisher: Elsevier B.V.
Citation: Mishra, Archita, Lai, Ghee Chuan, Yao, Leong Jing, Aung, Thet Tun, Shental, Noam, Rotter-Maskowitz, Aviva, Shepherdson, Edwin, Singh, Gurmit Singh Naranjan, Pai, Rhea, Shanti, Adhika, Wong, Regina Men Men, Lee, Andrea, Khyriem, Costerwell, Dutertre, Charles Antoine, Chakarov, Svetoslav, Srinivasan, K. G., Shadan, Nurhidaya Binte, Zhang, Xiao-Meng, Khalilnezhad, Shabnam, Cottier, Fabien, Tan, Alrina Shin Min, Low, Gillian, Chen, Phyllis, Fan, Yiping, Hor, Pei Xiang, Lee, Avery Khoo May, Choolani, Mahesh, Vermijlen, David, Sharma, Ankur, Fuks, Garold, Straussman, Ravid, Pavelka, Norman, Malleret, Benoit, McGovern, Naomi, Albani, Salvatore, Chan, Jerry Kok Yen, Ginhoux, Florent (2021-06-01). Microbial exposure during early human development primes fetal immune cells. Cell 184 (13). ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: The human fetal immune system begins to develop early during gestation; however, factors responsible for fetal immune-priming remain elusive. We explored potential exposure to microbial agents in utero and their contribution toward activation of memory T cells in fetal tissues. We profiled microbes across fetal organs using 16S rRNA gene sequencing and detected low but consistent microbial signal in fetal gut, skin, placenta, and lungs in the 2nd trimester of gestation. We identified several live bacterial strains including Staphylococcus and Lactobacillus in fetal tissues, which induced in vitro activation of memory T cells in fetal mesenteric lymph node, supporting the role of microbial exposure in fetal immune-priming. Finally, using SEM and RNA-ISH, we visualized discrete localization of bacteria-like structures and eubacterial-RNA within 14th weeks fetal gut lumen. These findings indicate selective presence of live microbes in fetal organs during the 2nd trimester of gestation and have broader implications toward the establishment of immune competency and priming before birth. © 2021
Source Title: Cell
ISSN: 0092-8674
DOI: 10.1016/j.cell.2021.04.039
Rights: Attribution 4.0 International
Appears in Collections:Staff Publications

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