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https://doi.org/10.1016/j.celrep.2021.109775
Title: | Diversity and function of motile ciliated cell types within ependymal lineages of the zebrafish brain | Authors: | D'Gama, Percival P. Qiu, Tao Cosacak, Mehmet Ilyas Rayamajhi, Dheeraj Konac, Ahsen Hansen, Jan Niklas Ringers, Christa Acuña-Hinrichsen, Francisca Hui, Subhra P. Olstad, Emilie W. Chong, Yan Ling Lim, Charlton Kang An Gupta, Astha Ng, Chee Peng Nilges, Benedikt S. Kashikar, Nachiket D. Wachten, Dagmar Liebl, David Kikuchi, Kazu Kizil, Caghan Yaksi, Emre Roy, Sudipto Jurisch-Yaksi, Nathalie |
Keywords: | brain cerebrospinal fluid choroid plexus cilia ependymal cell foxj1 gmnc multiciliated cells scoliosis zebrafish |
Issue Date: | 1-Oct-2021 | Publisher: | Elsevier B.V. | Citation: | D'Gama, Percival P., Qiu, Tao, Cosacak, Mehmet Ilyas, Rayamajhi, Dheeraj, Konac, Ahsen, Hansen, Jan Niklas, Ringers, Christa, Acuña-Hinrichsen, Francisca, Hui, Subhra P., Olstad, Emilie W., Chong, Yan Ling, Lim, Charlton Kang An, Gupta, Astha, Ng, Chee Peng, Nilges, Benedikt S., Kashikar, Nachiket D., Wachten, Dagmar, Liebl, David, Kikuchi, Kazu, Kizil, Caghan, Yaksi, Emre, Roy, Sudipto, Jurisch-Yaksi, Nathalie (2021-10-01). Diversity and function of motile ciliated cell types within ependymal lineages of the zebrafish brain. Cell Reports 37 (1) : 109775. ScholarBank@NUS Repository. https://doi.org/10.1016/j.celrep.2021.109775 | Rights: | Attribution-NonCommercial-NoDerivatives 4.0 International | Abstract: | Motile cilia defects impair cerebrospinal fluid (CSF) flow and can cause brain and spine disorders. The development of ciliated cells, their impact on CSF flow, and their function in brain and axial morphogenesis are not fully understood. We have characterized motile ciliated cells within the zebrafish brain ventricles. We show that the ventricles undergo restructuring through development, involving a transition from mono- to multiciliated cells (MCCs) driven by gmnc. MCCs co-exist with monociliated cells and generate directional flow patterns. These ciliated cells have different developmental origins and are genetically heterogenous with respect to expression of the Foxj1 family of ciliary master regulators. Finally, we show that cilia loss from the tela choroida and choroid plexus or global perturbation of multiciliation does not affect overall brain or spine morphogenesis but results in enlarged ventricles. Our findings establish that motile ciliated cells are generated by complementary and sequential transcriptional programs to support ventricular development. © 2021 The Author(s) | Source Title: | Cell Reports | URI: | https://scholarbank.nus.edu.sg/handle/10635/232799 | ISSN: | 2211-1247 | DOI: | 10.1016/j.celrep.2021.109775 | Rights: | Attribution-NonCommercial-NoDerivatives 4.0 International |
Appears in Collections: | Elements Staff Publications |
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