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Title: Aptamer laden liquid crystals biosensing platform for the detection of HIV-1 glycoprotein-120
Authors: Abbasi, Amna Didar
Hussain, Zakir
Yang, Kun-Lin 
Keywords: B40t77
Liquid crystals
RNA aptamer
Issue Date: 13-May-2021
Publisher: MDPI AG
Citation: Abbasi, Amna Didar, Hussain, Zakir, Yang, Kun-Lin (2021-05-13). Aptamer laden liquid crystals biosensing platform for the detection of HIV-1 glycoprotein-120. Molecules 26 (10) : 2893. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: We report a label-free and simple approach for the detection of glycoprotein-120 (gp-120) using an aptamer-based liquid crystals (LCs) biosensing platform. The LCs are supported on the surface of a modified glass slide with a suitable amount of B40t77 aptamer, allowing the LCs to be homeotropically aligned. A pronounced topological change was observed on the surface due to a specific interaction between B40t77 and gp-120, which led to the disruption of the homeotropic alignment of LCs. This results in a dark-to-bright transition observed under a polarized optical microscope. With the developed biosensing platform, it was possible to not only identify gp-120, but obtained results were analyzed quantitatively through image analysis. The detection limit of the proposed biosensing platform was investigated to be 0.2 µg/mL of gp-120. Regarding selectivity of the developed platform, no response could be detected when gp-120 was replaced by other proteins, such as bovine serum albumin (BSA), hepatitis A virus capsid protein 1 (Hep A VP1) and immunoglobulin G protein (IgG). Due to attributes such as label-free, high specificity and no need for instrumental read-out, the presented biosensing platform provides the potential to develop a working device for the quick detection of HIV-1 gp-120. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Source Title: Molecules
ISSN: 1420-3049
DOI: 10.3390/molecules26102893
Rights: Attribution 4.0 International
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