Please use this identifier to cite or link to this item:
https://doi.org/10.3390/molecules26102893
DC Field | Value | |
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dc.title | Aptamer laden liquid crystals biosensing platform for the detection of HIV-1 glycoprotein-120 | |
dc.contributor.author | Abbasi, Amna Didar | |
dc.contributor.author | Hussain, Zakir | |
dc.contributor.author | Yang, Kun-Lin | |
dc.date.accessioned | 2022-10-12T08:07:00Z | |
dc.date.available | 2022-10-12T08:07:00Z | |
dc.date.issued | 2021-05-13 | |
dc.identifier.citation | Abbasi, Amna Didar, Hussain, Zakir, Yang, Kun-Lin (2021-05-13). Aptamer laden liquid crystals biosensing platform for the detection of HIV-1 glycoprotein-120. Molecules 26 (10) : 2893. ScholarBank@NUS Repository. https://doi.org/10.3390/molecules26102893 | |
dc.identifier.issn | 1420-3049 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/232474 | |
dc.description.abstract | We report a label-free and simple approach for the detection of glycoprotein-120 (gp-120) using an aptamer-based liquid crystals (LCs) biosensing platform. The LCs are supported on the surface of a modified glass slide with a suitable amount of B40t77 aptamer, allowing the LCs to be homeotropically aligned. A pronounced topological change was observed on the surface due to a specific interaction between B40t77 and gp-120, which led to the disruption of the homeotropic alignment of LCs. This results in a dark-to-bright transition observed under a polarized optical microscope. With the developed biosensing platform, it was possible to not only identify gp-120, but obtained results were analyzed quantitatively through image analysis. The detection limit of the proposed biosensing platform was investigated to be 0.2 µg/mL of gp-120. Regarding selectivity of the developed platform, no response could be detected when gp-120 was replaced by other proteins, such as bovine serum albumin (BSA), hepatitis A virus capsid protein 1 (Hep A VP1) and immunoglobulin G protein (IgG). Due to attributes such as label-free, high specificity and no need for instrumental read-out, the presented biosensing platform provides the potential to develop a working device for the quick detection of HIV-1 gp-120. © 2021 by the authors. Licensee MDPI, Basel, Switzerland. | |
dc.publisher | MDPI AG | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.source | Scopus OA2021 | |
dc.subject | B40t77 | |
dc.subject | Biosensor | |
dc.subject | DMOAP | |
dc.subject | Gp-120 | |
dc.subject | Liquid crystals | |
dc.subject | RNA aptamer | |
dc.type | Article | |
dc.contributor.department | COLLEGE OF DESIGN AND ENGINEERING | |
dc.description.doi | 10.3390/molecules26102893 | |
dc.description.sourcetitle | Molecules | |
dc.description.volume | 26 | |
dc.description.issue | 10 | |
dc.description.page | 2893 | |
Appears in Collections: | Elements Staff Publications |
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