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Title: High-dose drug heat map analysis for drug safety and efficacy in multi-spheroid brain normal cells and GBM patient-derived cells
Authors: Lee, Sang-Yun
Teng, Yvonne
Son, Miseol
Ku, Bosung
Moon, Ho Sang
Tergaonkar, Vinay 
Chow, Pierce Kah-Hoe 
Lee, Dong Woo
Nam, Do-Hyun
Issue Date: 2-Dec-2021
Publisher: Public Library of Science
Citation: Lee, Sang-Yun, Teng, Yvonne, Son, Miseol, Ku, Bosung, Moon, Ho Sang, Tergaonkar, Vinay, Chow, Pierce Kah-Hoe, Lee, Dong Woo, Nam, Do-Hyun (2021-12-02). High-dose drug heat map analysis for drug safety and efficacy in multi-spheroid brain normal cells and GBM patient-derived cells. PLoS ONE 16 (12-Dec) : e0251998. ScholarBank@NUS Repository.
Rights: Attribution 4.0 International
Abstract: To test the safety and efficacy of drugs via a high does drug heat map, a multi-spheroids array chip was developed by adopting a micropillar and microwell structure. In the chip, patient-derived cells were encapsulated in alginate and grown to maturity for more than 7 days to form cancer multi-spheroids. Multi-spheroids grown in conventional well plates require many cells and are easily damaged as a result of multiple pipetting during maintenance culture or experimental procedures. To address these issues, we applied a micropillar and microwell structure to the multi-spheroids array. Patient-derived cells from patients with Glioblastoma (GBM), the most common and lethal form of central nervous system cancer, were used to validate the array chip performance. After forming multi-spheroids with a diameter greater than 100?m in a 12×36 pillar array chip (25mm × 75mm), we tested 70 drug compounds (6 replicates) using a high-dose to determine safety and efficacy for drug candidates. Comparing the drug response of multi-spheroids derived from normal cells and cancer cells, we found that four compounds (Dacomitinib, Cediranib, LY2835219, BGJ398) did not show toxicity to astrocyte cell and were efficacious to patient-derived GBM cells. Copyright: © 2021 Lee et al.
Source Title: PLoS ONE
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0251998
Rights: Attribution 4.0 International
Appears in Collections:Staff Publications

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