Please use this identifier to cite or link to this item: https://doi.org/10.1002/eji.202170126
Title: Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)
Authors: Cossarizza, Andrea
Chang, Hyun-Dong
Radbruch, Andreas
Abrignani, Sergio
Addo, Richard
Akdis, Muebeccel
Andrä, I.
Andreata, Francesco
Annunziato, Francesco
Arranz, Eduardo
Bacher, Petra
Lenz, Daniel
Levings, Megan K.
Lino, Andreia C.
Liotta, Francesco
Long, Heather M.
Lugli, Enrico
MacDonald, Katherine N.
Maggi, Laura
Maini, Mala K.
Mair, Florian
Bari, Sudipto 
Manta, Calin
Manz, Rudolf Armin
Mashreghi, Mir-Farzin
Mazzoni, Alessio
McCluskey, James
Mei, Henrik E.
Melchers, Fritz
Melzer, Susanne
Mielenz, Dirk
Monin, Leticia
Barnaba, Vincenzo
Moretta, Lorenzo
Multhoff, Gabriele
Muñoz, L.E.
Muñoz-Ruiz, M.
Muscate, Franziska
Natalini, Ambra
Neumann, Katrin
Ng, Lai Guan 
Niedobitek, Antonia
Niemz, Jana
Barros-Martins, Joana
Almeida, Larissa Nogueira
Notarbartolo, Samuele
Ostendorf, Lennard
Pallett, Laura J.
Patel, Amit A.
Percin, Gulce Itir
Peruzzi, Giovanna
Pinti, Marcello
Pockley, A. Graham
Pracht, Katharina
Baumjohann, Dirk
Prinz, Immo
Pujol-Autonell, Irma
Pulvirenti, Nadia
Quatrini, Linda
Quinn, Kylie M.
Radbruch, Helena
Rhys, Hefin
Rodrigo, Maria B.
Romagnani, Chiara
Saggau, Carina
Beccaria, Cristian G.
Sakaguchi, Shimon
Sallusto, Federica
Sanderink, Lieke
Sandrock, Inga
Schauer, Christine
Scheffold, Alexander
Scherer, Hans U.
Schiemann, Matthias
Schildberg, Frank A.
Schober, Kilian
Bernardo, David
Schoen, Janina
Schuh, Wolfgang
Schüler, T.
Schulz, Axel R.
Schulz, Sebastian
Schulze, Julia
Simonetti, Sonia
Singh, Jeeshan
Sitnik, Katarzyna M.
Stark, Regina
Boardman, Dominic A.
Starossom, Sarah
Stehle, Christina
Szelinski, Franziska
Tan, Leonard
Tarnok, Attila
Tornack, Julia
Tree, Timothy I. M.
van Beek, Jasper J. P.
van de Veen, Willem
van Gisbergen, Klaas
Borger, Jessica
Vasco, Chiara
Verheyden, Nikita A.
von Borstel, Anouk
Ward-Hartstonge, Kirsten A.
Warnatz, Klaus
Waskow, Claudia
Wiedemann, Annika
Wilharm, Anneke
Wing, James
Wirz, Oliver
Böttcher, C.
Wittner, Jens
Yang, Jennie H. M.
Yang, Juhao
Brockmann, Leonie
Burns, Marie
Busch, Dirk H.
Cameron, Garth
Cammarata, Ilenia
Cassotta, Antonino
Chang, Yinshui
Chirdo, Fernando Gabriel
Christakou, Eleni
Čičin-Šain, L.
Cook, Laura
Corbett, Alexandra J.
Cornelis, Rebecca
Cosmi, Lorenzo
Davey, Martin S.
De Biasi, Sara
De Simone, Gabriele
del Zotto, Genny
Delacher, Michael
Di Rosa, Francesca
Santo, J.D.
Diefenbach, Andreas
Dong, Jun
Dörner, T.
Dress, Regine J.
Dutertre, Charles-Antoine
Eckle, Sidonia B. G.
Eede, Pascale
Evrard, Maximilien
Falk, Christine S.
Feuerer, Markus
Fillatreau, Simon
Fiz-Lopez, Aida
Follo, Marie
Foulds, Gemma A.
Fröbel, J.
Gagliani, Nicola
Galletti, Giovanni
Gangaev, Anastasia
Garbi, Natalio
Garrote, Jose Antonio
Geginat, Jens
Gherardin, Nicholas A.
Gibellini, Lara
Ginhoux, Florent 
Godfrey, Dale I.
Gruarin, Paola
Haftmann, Claudia
Hansmann, Leo
Harpur, Christopher M.
Hayday, Adrian C.
Heine, Guido
Hernández, D.C.
Herrmann, Martin
Hoelsken, Oliver
Huang, Qing
Huber, Samuel
Huber, Johanna E.
Huehn, Jochen
Hundemer, Michael
Hwang, William Y. K. 
Iannacone, Matteo
Ivison, Sabine M.
Jäck, H.-M.
Jani, Peter K.
Keller, Baerbel
Kessler, Nina
Ketelaars, Steven
Knop, Laura
Knopf, Jasmin
Koay, Hui-Fern
Kobow, Katja
Kriegsmann, Katharina
Kristyanto, H.
Krueger, Andreas
Kuehne, Jenny F.
Kunze-Schumacher, Heike
Kvistborg, Pia
Kwok, Immanuel
Latorre, Daniela
Issue Date: 1-Dec-2021
Publisher: John Wiley and Sons Inc
Citation: Cossarizza, Andrea, Chang, Hyun-Dong, Radbruch, Andreas, Abrignani, Sergio, Addo, Richard, Akdis, Muebeccel, Andrä, I., Andreata, Francesco, Annunziato, Francesco, Arranz, Eduardo, Bacher, Petra, Lenz, Daniel, Levings, Megan K., Lino, Andreia C., Liotta, Francesco, Long, Heather M., Lugli, Enrico, MacDonald, Katherine N., Maggi, Laura, Maini, Mala K., Mair, Florian, Bari, Sudipto, Manta, Calin, Manz, Rudolf Armin, Mashreghi, Mir-Farzin, Mazzoni, Alessio, McCluskey, James, Mei, Henrik E., Melchers, Fritz, Melzer, Susanne, Mielenz, Dirk, Monin, Leticia, Barnaba, Vincenzo, Moretta, Lorenzo, Multhoff, Gabriele, Muñoz, L.E., Muñoz-Ruiz, M., Muscate, Franziska, Natalini, Ambra, Neumann, Katrin, Ng, Lai Guan, Niedobitek, Antonia, Niemz, Jana, Barros-Martins, Joana, Almeida, Larissa Nogueira, Notarbartolo, Samuele, Ostendorf, Lennard, Pallett, Laura J., Patel, Amit A., Percin, Gulce Itir, Peruzzi, Giovanna, Pinti, Marcello, Pockley, A. Graham, Pracht, Katharina, Baumjohann, Dirk, Prinz, Immo, Pujol-Autonell, Irma, Pulvirenti, Nadia, Quatrini, Linda, Quinn, Kylie M., Radbruch, Helena, Rhys, Hefin, Rodrigo, Maria B., Romagnani, Chiara, Saggau, Carina, Beccaria, Cristian G., Sakaguchi, Shimon, Sallusto, Federica, Sanderink, Lieke, Sandrock, Inga, Schauer, Christine, Scheffold, Alexander, Scherer, Hans U., Schiemann, Matthias, Schildberg, Frank A., Schober, Kilian, Bernardo, David, Schoen, Janina, Schuh, Wolfgang, Schüler, T., Schulz, Axel R., Schulz, Sebastian, Schulze, Julia, Simonetti, Sonia, Singh, Jeeshan, Sitnik, Katarzyna M., Stark, Regina, Boardman, Dominic A., Starossom, Sarah, Stehle, Christina, Szelinski, Franziska, Tan, Leonard, Tarnok, Attila, Tornack, Julia, Tree, Timothy I. M., van Beek, Jasper J. P., van de Veen, Willem, van Gisbergen, Klaas, Borger, Jessica, Vasco, Chiara, Verheyden, Nikita A., von Borstel, Anouk, Ward-Hartstonge, Kirsten A., Warnatz, Klaus, Waskow, Claudia, Wiedemann, Annika, Wilharm, Anneke, Wing, James, Wirz, Oliver, Böttcher, C., Wittner, Jens, Yang, Jennie H. M., Yang, Juhao, Brockmann, Leonie, Burns, Marie, Busch, Dirk H., Cameron, Garth, Cammarata, Ilenia, Cassotta, Antonino, Chang, Yinshui, Chirdo, Fernando Gabriel, Christakou, Eleni, Čičin-Šain, L., Cook, Laura, Corbett, Alexandra J., Cornelis, Rebecca, Cosmi, Lorenzo, Davey, Martin S., De Biasi, Sara, De Simone, Gabriele, del Zotto, Genny, Delacher, Michael, Di Rosa, Francesca, Santo, J.D., Diefenbach, Andreas, Dong, Jun, Dörner, T., Dress, Regine J., Dutertre, Charles-Antoine, Eckle, Sidonia B. G., Eede, Pascale, Evrard, Maximilien, Falk, Christine S., Feuerer, Markus, Fillatreau, Simon, Fiz-Lopez, Aida, Follo, Marie, Foulds, Gemma A., Fröbel, J., Gagliani, Nicola, Galletti, Giovanni, Gangaev, Anastasia, Garbi, Natalio, Garrote, Jose Antonio, Geginat, Jens, Gherardin, Nicholas A., Gibellini, Lara, Ginhoux, Florent, Godfrey, Dale I., Gruarin, Paola, Haftmann, Claudia, Hansmann, Leo, Harpur, Christopher M., Hayday, Adrian C., Heine, Guido, Hernández, D.C., Herrmann, Martin, Hoelsken, Oliver, Huang, Qing, Huber, Samuel, Huber, Johanna E., Huehn, Jochen, Hundemer, Michael, Hwang, William Y. K., Iannacone, Matteo, Ivison, Sabine M., Jäck, H.-M., Jani, Peter K., Keller, Baerbel, Kessler, Nina, Ketelaars, Steven, Knop, Laura, Knopf, Jasmin, Koay, Hui-Fern, Kobow, Katja, Kriegsmann, Katharina, Kristyanto, H., Krueger, Andreas, Kuehne, Jenny F., Kunze-Schumacher, Heike, Kvistborg, Pia, Kwok, Immanuel, Latorre, Daniela (2021-12-01). Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition). European Journal of Immunology 51 (12) : 2708-3145. ScholarBank@NUS Repository. https://doi.org/10.1002/eji.202170126
Rights: Attribution 4.0 International
Abstract: The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine immune cell subsets. Notably, the Guidelines contain helpful tables highlighting phenotypes and key differences between human and murine cells. Another useful feature of this edition is the flow cytometry analysis of clinical samples with examples of flow cytometry applications in the context of autoimmune diseases, cancers as well as acute and chronic infectious diseases. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid. All sections are written and peer-reviewed by leading flow cytometry experts and immunologists, making this edition an essential and state-of-the-art handbook for basic and clinical researchers. © 2021 Wiley-VCH GmbH
Source Title: European Journal of Immunology
URI: https://scholarbank.nus.edu.sg/handle/10635/232192
ISSN: 0014-2980
DOI: 10.1002/eji.202170126
Rights: Attribution 4.0 International
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