Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.jchromb.2006.07.062
Title: Simple reversed-phase liquid chromatographic assay for simultaneous quantification of free mycophenolic acid and its glucuronide metabolite in human plasma
Authors: Yau, Wai-Ping 
Vathsala, Anantharaman 
Lou, Huei-Xin
Chan, Eli 
Keywords: Science & Technology
Life Sciences & Biomedicine
Physical Sciences
Biochemical Research Methods
Chemistry, Analytical
Biochemistry & Molecular Biology
Chemistry
mycophenolic acid
mycophenolic acid glucuronide
reversed-phase HPLC
free drug
human plasma
TANDEM MASS-SPECTROMETRY
TRANSPLANT PATIENTS
RENAL-TRANSPLANT
PHARMACOKINETICS
MOFETIL
RECIPIENTS
THERAPY
URINE
SERUM
Issue Date: 1-Feb-2007
Publisher: ELSEVIER SCIENCE BV
Citation: Yau, Wai-Ping, Vathsala, Anantharaman, Lou, Huei-Xin, Chan, Eli (2007-02-01). Simple reversed-phase liquid chromatographic assay for simultaneous quantification of free mycophenolic acid and its glucuronide metabolite in human plasma. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 846 (1-2) : 313-318. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jchromb.2006.07.062
Abstract: A reversed-phase HPLC-UV method, involving simple instrumental setup and mobile phase without ion-pairing reagent, was developed and validated for direct simultaneous quantification of free mycophenolic acid (MPA) and its major metabolite MPA-glucuronide (MPAG) in human plasma. Both free MPA and MPAG were isolated from plasma samples using ultrafiltration prior to analysis. Each chromatographic run was completed within 13 min. The optimized method showed good performance in terms of specificity, linearity (r2 = 0.9999), sensitivity (limit of quantitation (LOQ): 0.005 mg/L for MPA; 1 mg/L for MPAG), and intra- and inter-day precision (R.S.D. < 7%). This assay was successfully applied to free MPA and MPAG measurements in clinical samples. © 2006 Elsevier B.V. All rights reserved.
Source Title: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
URI: https://scholarbank.nus.edu.sg/handle/10635/229451
ISSN: 1570-0232
1873-376X
DOI: 10.1016/j.jchromb.2006.07.062
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