Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.jchromb.2006.07.062
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dc.titleSimple reversed-phase liquid chromatographic assay for simultaneous quantification of free mycophenolic acid and its glucuronide metabolite in human plasma
dc.contributor.authorYau, Wai-Ping
dc.contributor.authorVathsala, Anantharaman
dc.contributor.authorLou, Huei-Xin
dc.contributor.authorChan, Eli
dc.date.accessioned2022-07-29T07:09:35Z
dc.date.available2022-07-29T07:09:35Z
dc.date.issued2007-02-01
dc.identifier.citationYau, Wai-Ping, Vathsala, Anantharaman, Lou, Huei-Xin, Chan, Eli (2007-02-01). Simple reversed-phase liquid chromatographic assay for simultaneous quantification of free mycophenolic acid and its glucuronide metabolite in human plasma. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 846 (1-2) : 313-318. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jchromb.2006.07.062
dc.identifier.issn1570-0232
dc.identifier.issn1873-376X
dc.identifier.urihttps://scholarbank.nus.edu.sg/handle/10635/229451
dc.description.abstractA reversed-phase HPLC-UV method, involving simple instrumental setup and mobile phase without ion-pairing reagent, was developed and validated for direct simultaneous quantification of free mycophenolic acid (MPA) and its major metabolite MPA-glucuronide (MPAG) in human plasma. Both free MPA and MPAG were isolated from plasma samples using ultrafiltration prior to analysis. Each chromatographic run was completed within 13 min. The optimized method showed good performance in terms of specificity, linearity (r2 = 0.9999), sensitivity (limit of quantitation (LOQ): 0.005 mg/L for MPA; 1 mg/L for MPAG), and intra- and inter-day precision (R.S.D. < 7%). This assay was successfully applied to free MPA and MPAG measurements in clinical samples. © 2006 Elsevier B.V. All rights reserved.
dc.language.isoen
dc.publisherELSEVIER SCIENCE BV
dc.sourceElements
dc.subjectScience & Technology
dc.subjectLife Sciences & Biomedicine
dc.subjectPhysical Sciences
dc.subjectBiochemical Research Methods
dc.subjectChemistry, Analytical
dc.subjectBiochemistry & Molecular Biology
dc.subjectChemistry
dc.subjectmycophenolic acid
dc.subjectmycophenolic acid glucuronide
dc.subjectreversed-phase HPLC
dc.subjectfree drug
dc.subjecthuman plasma
dc.subjectTANDEM MASS-SPECTROMETRY
dc.subjectTRANSPLANT PATIENTS
dc.subjectRENAL-TRANSPLANT
dc.subjectPHARMACOKINETICS
dc.subjectMOFETIL
dc.subjectRECIPIENTS
dc.subjectTHERAPY
dc.subjectURINE
dc.subjectSERUM
dc.typeArticle
dc.date.updated2022-07-23T13:40:12Z
dc.contributor.departmentDEPT OF PHARMACY
dc.contributor.departmentMEDICINE
dc.contributor.departmentPHARMACY
dc.description.doi10.1016/j.jchromb.2006.07.062
dc.description.sourcetitleJOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
dc.description.volume846
dc.description.issue1-2
dc.description.page313-318
dc.published.statePublished
dc.description.redepositcompleted
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