Please use this identifier to cite or link to this item: https://doi.org/10.1038/s41467-021-25004-9
Title: Quantifying nanodiamonds biodistribution in whole cells with correlative iono-nanoscopy
Authors: Mi, Zhaohong 
Chen, Ce-Belle 
Tan, Hong Q
Dou, Yanxin 
Yang, Chengyuan 
Turaga, Shuvan Prashant 
Ren, Minqin 
Vajandar, Saumitra K 
Yuen, Gin Hao 
Osipowicz, Thomas 
Watt, Frank 
Bettiol, Andrew A 
Keywords: Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
NITROGEN-VACANCY CENTERS
ELECTRON-MICROSCOPY
LIGHT
TRACKING
Issue Date: 2-Aug-2021
Publisher: NATURE PORTFOLIO
Citation: Mi, Zhaohong, Chen, Ce-Belle, Tan, Hong Q, Dou, Yanxin, Yang, Chengyuan, Turaga, Shuvan Prashant, Ren, Minqin, Vajandar, Saumitra K, Yuen, Gin Hao, Osipowicz, Thomas, Watt, Frank, Bettiol, Andrew A (2021-08-02). Quantifying nanodiamonds biodistribution in whole cells with correlative iono-nanoscopy. NATURE COMMUNICATIONS 12 (1). ScholarBank@NUS Repository. https://doi.org/10.1038/s41467-021-25004-9
Abstract: Correlative imaging and quantification of intracellular nanoparticles with the underlying ultrastructure is crucial for understanding cell-nanoparticle interactions in biological research. However, correlative nanoscale imaging of whole cells still remains a daunting challenge. Here, we report a straightforward nanoscopic approach for whole-cell correlative imaging, by simultaneous ionoluminescence and ultrastructure mapping implemented with a highly focused beam of alpha particles. We demonstrate that fluorescent nanodiamonds exhibit fast, ultrabright and stable emission upon excitation by alpha particles. Thus, by using fluorescent nanodiamonds as imaging probes, our approach enables quantification and correlative localization of single nanodiamonds within a whole cell at sub-30 nm resolution. As an application example, we show that our approach, together with Monte Carlo simulations and radiobiological experiments, can be employed to provide unique insights into the mechanisms of nanodiamond radiosensitization at the single whole-cell level. These findings may benefit clinical studies of radio-enhancement effects by nanoparticles in charged-particle cancer therapy.
Source Title: NATURE COMMUNICATIONS
URI: https://scholarbank.nus.edu.sg/handle/10635/228885
ISSN: 2041-1723
DOI: 10.1038/s41467-021-25004-9
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