Please use this identifier to cite or link to this item: https://doi.org/10.1038/s41598-020-79820-y
Title: IgE-binding residues analysis of the house dust mite allergen Der p 23
Authors: Pang, Sze Lei 
Matta, Sri Anusha
Sio, Yang Yie 
Ng, Yu Ting 
Say, Yee-How
Ng, Chyan Leong
Chew, Fook Tim 
Keywords: Science & Technology
Multidisciplinary Sciences
Science & Technology - Other Topics
MAGNETIC-RESONANCE STRUCTURE
BLOMIA-TROPICALIS
EPITOPES
IDENTIFICATION
ASTHMA
SENSITIZATION
ANTIBODIES
CHILDREN
RHINITIS
POLLEN
Issue Date: 13-Jan-2021
Publisher: NATURE PORTFOLIO
Citation: Pang, Sze Lei, Matta, Sri Anusha, Sio, Yang Yie, Ng, Yu Ting, Say, Yee-How, Ng, Chyan Leong, Chew, Fook Tim (2021-01-13). IgE-binding residues analysis of the house dust mite allergen Der p 23. SCIENTIFIC REPORTS 11 (1). ScholarBank@NUS Repository. https://doi.org/10.1038/s41598-020-79820-y
Abstract: House dust mites (HDMs) are one of the major causes of allergies in the world. The group 23 allergen, Der p 23, from Dermatophagoides pteronyssinus, is a major allergen amongst HDM-sensitized individuals. This study aims to determine the specific immunoglobulin E (sIgE) binding frequency and IgE-binding residues of recombinant Der p 23 (rDer p 23) allergen amongst a cohort of consecutive atopic individuals in a tropical region. We performed site-directed mutagenesis and carried out immuno-dot blot assays using 65 atopic sera. The immuno-dot blot assays results indicated that the two residues K44 and E46 which are located at the N-terminal region are the major IgE-binding residues. The rDerp-23 sIgE titers are strongly correlated to the number of IgE-binding residues for rDer p 23 (P < 0.001). Atopic individuals who were only sensitized to HDM have a significantly higher number of IgE-binding residues than the individuals who were polysensitized to HDM and other crude allergens (P < 0.05). Individuals with allergic multimorbidity and moderate-to-severe allergic rhinitis also have a higher number of IgE-binding residues compared to those with single allergic disease and mild allergic rhinitis. The results prompt us to hypothesize that the individuals who have a higher number of IgE-binding residues may face a bigger challenge to be treated through immunotherapy due to the complexity in designing an effective hypoallergen with a high number of IgE-binding residues. We propose that the development of a refined molecular diagnostic assay, which includes alanine substitution of surface-exposed residues could be a more precise diagnostic strategy to identify all the IgE-binding residues of a major allergen for an atopic individual and the development could be another new dimension in allergy diagnosis and allergen immunotherapy treatment.
Source Title: SCIENTIFIC REPORTS
URI: https://scholarbank.nus.edu.sg/handle/10635/227038
ISSN: 2045-2322
DOI: 10.1038/s41598-020-79820-y
Appears in Collections:Staff Publications
Elements

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
IgE-binding residues analysis of the house dust mite allergen Der p 23.pdf2.03 MBAdobe PDF

OPEN

PublishedView/Download

SCOPUSTM   
Citations

2
checked on Sep 23, 2022

Page view(s)

35
checked on Sep 22, 2022

Download(s)

1
checked on Sep 22, 2022

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.