Please use this identifier to cite or link to this item:
https://doi.org/10.1038/s41598-020-79820-y
DC Field | Value | |
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dc.title | IgE-binding residues analysis of the house dust mite allergen Der p 23 | |
dc.contributor.author | Pang, Sze Lei | |
dc.contributor.author | Matta, Sri Anusha | |
dc.contributor.author | Sio, Yang Yie | |
dc.contributor.author | Ng, Yu Ting | |
dc.contributor.author | Say, Yee-How | |
dc.contributor.author | Ng, Chyan Leong | |
dc.contributor.author | Chew, Fook Tim | |
dc.date.accessioned | 2022-06-13T09:03:52Z | |
dc.date.available | 2022-06-13T09:03:52Z | |
dc.date.issued | 2021-01-13 | |
dc.identifier.citation | Pang, Sze Lei, Matta, Sri Anusha, Sio, Yang Yie, Ng, Yu Ting, Say, Yee-How, Ng, Chyan Leong, Chew, Fook Tim (2021-01-13). IgE-binding residues analysis of the house dust mite allergen Der p 23. SCIENTIFIC REPORTS 11 (1). ScholarBank@NUS Repository. https://doi.org/10.1038/s41598-020-79820-y | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | https://scholarbank.nus.edu.sg/handle/10635/227038 | |
dc.description.abstract | House dust mites (HDMs) are one of the major causes of allergies in the world. The group 23 allergen, Der p 23, from Dermatophagoides pteronyssinus, is a major allergen amongst HDM-sensitized individuals. This study aims to determine the specific immunoglobulin E (sIgE) binding frequency and IgE-binding residues of recombinant Der p 23 (rDer p 23) allergen amongst a cohort of consecutive atopic individuals in a tropical region. We performed site-directed mutagenesis and carried out immuno-dot blot assays using 65 atopic sera. The immuno-dot blot assays results indicated that the two residues K44 and E46 which are located at the N-terminal region are the major IgE-binding residues. The rDerp-23 sIgE titers are strongly correlated to the number of IgE-binding residues for rDer p 23 (P < 0.001). Atopic individuals who were only sensitized to HDM have a significantly higher number of IgE-binding residues than the individuals who were polysensitized to HDM and other crude allergens (P < 0.05). Individuals with allergic multimorbidity and moderate-to-severe allergic rhinitis also have a higher number of IgE-binding residues compared to those with single allergic disease and mild allergic rhinitis. The results prompt us to hypothesize that the individuals who have a higher number of IgE-binding residues may face a bigger challenge to be treated through immunotherapy due to the complexity in designing an effective hypoallergen with a high number of IgE-binding residues. We propose that the development of a refined molecular diagnostic assay, which includes alanine substitution of surface-exposed residues could be a more precise diagnostic strategy to identify all the IgE-binding residues of a major allergen for an atopic individual and the development could be another new dimension in allergy diagnosis and allergen immunotherapy treatment. | |
dc.language.iso | en | |
dc.publisher | NATURE PORTFOLIO | |
dc.source | Elements | |
dc.subject | Science & Technology | |
dc.subject | Multidisciplinary Sciences | |
dc.subject | Science & Technology - Other Topics | |
dc.subject | MAGNETIC-RESONANCE STRUCTURE | |
dc.subject | BLOMIA-TROPICALIS | |
dc.subject | EPITOPES | |
dc.subject | IDENTIFICATION | |
dc.subject | ASTHMA | |
dc.subject | SENSITIZATION | |
dc.subject | ANTIBODIES | |
dc.subject | CHILDREN | |
dc.subject | RHINITIS | |
dc.subject | POLLEN | |
dc.type | Article | |
dc.date.updated | 2022-06-10T02:32:54Z | |
dc.contributor.department | DEAN'S OFFICE (SCIENCE) | |
dc.contributor.department | BIOLOGICAL SCIENCES | |
dc.description.doi | 10.1038/s41598-020-79820-y | |
dc.description.sourcetitle | SCIENTIFIC REPORTS | |
dc.description.volume | 11 | |
dc.description.issue | 1 | |
dc.published.state | Published | |
Appears in Collections: | Staff Publications Elements |
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IgE-binding residues analysis of the house dust mite allergen Der p 23.pdf | 2.03 MB | Adobe PDF | OPEN | Published | View/Download |
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